Evidence map›Paper›PMID 40321999›Full record

ArticleBiomedical optics express2025

Zebrafish fluorescence imaging platform based on Bessel light sheet illumination.

Chuhui Wang, Dongmei Su, Ziheng Zhang, Jiaju Chen, Yang Liu, Cuiyi Peng, Yachen Fan, Chenggang Yan, Sanyang Han, Minjiang Chen and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Chuhui WangPrecision Medicine and Public Health, Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen, Guangdong 518055, China.
Dongmei SuDepartment of Biology, Southern University of Science and Technology, Shenzhen, China.
Ziheng ZhangInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Jiaju ChenInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Yang LiuInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Cuiyi PengInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Yachen FanInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Chenggang YanSchool of Automation, Hangzhou Dianzi University, Hangzhou, Zhejiang Province 310018, China.
Sanyang HanInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Minjiang ChenZhejiang Key Laboratory of Imaging and Interventional Medicine, Zhejiang Engineering Research Center of Interventional Medicine Engineering and Biotechnology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, China.
Xingru HuangSchool of Automation, Hangzhou Dianzi University, Hangzhou, Zhejiang Province 310018, China.
Jiansong JiZhejiang Key Laboratory of Imaging and Interventional Medicine, Zhejiang Engineering Research Center of Interventional Medicine Engineering and Biotechnology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, China.
Zhenglin ChenInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.
Dong LiuDepartment of Biology, Southern University of Science and Technology, Shenzhen, China.
Dongmei YuSchool of Mechanical, Electrical & Information Engineering, Shandong University, Weihai, Shandong 264209, China.
Peiwu QinInstitute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong 518055, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a three-dimensional (3D) zebrafish fluorescence imaging platform based on Bessel light sheet fluorescence microscopy (LSFM). During the 3D imaging process, the excitation light sheet remains static and the axial scanning is realized by moving the sample with one motorized positioning stage. To solve the defocusing problem caused by the optical path length change in 3D imaging, an electrically tunable lens (ETL) is adopted in the detection optical path. An auto-refocusing method that considers the sample structural anisotropy and has no limitation on the mathematical form of signals added to the ETL is designed. The results show that ETL can provide a satisfactory refocusing effect using detection objectives with a low numerical aperture (NA). In addition, the effects of the ETL on the system magnification and resolution are explored. A magnification calibration method is devised to refine the precision of the volume synthesis. The system design also facilitates the recording of ambient noise, which can help improve image quality with simple background image subtraction. This hardware-based background elimination method is compared with several state-of-the-art fluorescence image denoising algorithms, and the comparison results verified the high performance of this method. The imaging results of live zebrafish lymphatic and vascular structures, as well as blood flow, prove the reliability of this platform without necessitating further image deconvolution.

Identifiers

PMID40321999
PMCPMC12047710

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