ArticleInternational journal of general medicine2025
Comparative Efficacy of Swim-Up, Density-Gradient Centrifugation, and Microfluidic Sorting in Sperm Preparation, and the Impact on Motility, Morphology, and DNA Integrity.
Article in International journal of general medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparative analysis of sperm preparation techniques on DNA fragmentation and clinical outcomes: a network meta-analysis.Frontiers in endocrinology · 2026Pooled it
- Spinal Cord Injury-Related Male Infertility: Oxidative Stress, Inflammation, and Sperm DNA Damage.Journal of clinical medicine · 2026Review
- Reactive Oxygen Species-Induced Damage in Cryopreserved Bovine Sperm: Mitigation Strategies.Veterinary medicine and science · 2026Review
- Evaluating the potential for sperm DNA fragmentation testing to guide the use of ICSI for couples with non-severe male infertility.Human reproduction open · 2026Article
Corrections and comments
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Authors and funding
9 authors.
Funding
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Abstract
Background: Reactive oxygen species (ROS) are considered a major factor contributing to sperm DNA damage during sperm preparation for assisted reproductive technologies (ART). This study aimed to investigate whether microfluidic sorting can select sperm with a low DNA fragmentation index (DFI) and to explore the underlying mechanisms. We compared the effects of three sperm preparation methods-swim-up, density-gradient centrifugation, and microfluidic sorting-on sperm quality and DNA integrity. Methods: Semen samples from 12 patients were divided into three equal portions and processed using swim-up, density-gradient centrifugation, and microfluidic sorting techniques. Sperm concentration, motility, morphology, DFI, intracellular H Results: Sperm prepared using microfluidic sorting exhibited significantly higher total motility (85.3 ± 3.2%) and progressive forward motility (72.5 ± 2.8%) compared to density-gradient centrifugation (total motility: 70.1 ± 3.5%; progressive motility: 58.4 ± 3.1%). Microfluidic sorting also resulted in a significantly lower DFI (8.2 ± 1.5%) compared to density-gradient centrifugation (25.6 ± 2.3%) and swim-up (15.4 ± 1.8%). Intracellular H Conclusion: Microfluidic sorting is an effective method for selecting sperm with higher motility, normal morphology, and lower DFI, while also reducing mitochondrial O
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.