Evidence map›Paper›PMID 40321540›Full record

ArticleACS omega2025

Improved Frontal Gel Filtration Chromatography to Examine the Interaction between Small Molecules and a Protein with Multiple Specific Binding Sites.

Marie Yamauchi, Hiromasa Tojo, Takemitsu Arakaki, Tetsuo Ishida

Abstract read
In one paragraph

Article in ACS omega, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marie YamauchiGraduate School of Engineering and Science, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Nakagami-gun 903-0213, Okinawa, Japan.
Hiromasa TojoOmniSeparo-TJ, Inc, Amagasaki 660-0083, Hyogo, Japan.
Takemitsu ArakakiDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Nakagami-gun 903-0213, Okinawa, Japan.
Tetsuo IshidaDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Nakagami-gun 903-0213, Okinawa, Japan.ORCID https://orcid.org/0009-0009-5150-9433

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Interaction between small molecules (ligands) and a protein is the basis of protein function and drug action. Therefore, it is essential to know the number and binding constants of specific binding sites that are most relevant to protein functions. These binding parameters can be estimated by the analysis of the average number of bound ligands per protein (

Identifiers

PMID40321540
PMCPMC12044467

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.