Evidence map›Paper›PMID 40319256›Full record

ArticleBMC plant biology2025

Identification and characterization of the powdery mildew resistance in cultivated emmer wheat accession Lxd-682 via bulked segregant RNA sequencing.

Jiatong Li, Ningning Yu, Nina Sun, Lige Geng, Yanmin Qie, Dongfeng Zhai, Yuxiang Wang, Linzhi Li, Xueqing Liu, Xusheng Sun and 7 more

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Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

17 authors.

Jiatong Li *Yantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China.
Ningning Yu *Yantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China.
Nina SunYantai Academy of Agricultural Sciences, Yantai, 265500, China.
Lige GengInstitute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Crop Genetic and Breeding, Shijiazhuang, 050035, China.
Yanmin QieInstitute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Crop Genetic and Breeding, Shijiazhuang, 050035, China.
Dongfeng ZhaiShandong Denghai Seeds Co., Ltd, Laizhou, 261448, China.
Yuxiang WangShandong Denghai Seeds Co., Ltd, Laizhou, 261448, China.
Linzhi LiYantai Academy of Agricultural Sciences, Yantai, 265500, China.
Xueqing LiuYantai Academy of Agricultural Sciences, Yantai, 265500, China.
Xusheng SunYantai Agricultural Technology Extension Center, Yantai, 264001, China.
Jiangchun WangYantai Academy of Agricultural Sciences, Yantai, 265500, China.
Ruishan LiuYantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China.
Guantong PanYantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China.
Shengmao ZouYantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China.
Guohao HanCenter for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China.
Yuli JinYantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China. yulijin@ytu.edu.cn.
Pengtao MaYantai Key Laboratory of Characteristic Agricultural Biological Resources Conservation and Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, 264005, China. ptma@ytu.edu.cn.

Funding

Key R&D Program of Shandong Province 2024LZGC001National Modern Wheat Industry Technology System, Yantai Comprehensive Experimental Station project CARS-03-62Natural Science Foundation of Hebei Province C2023503014Science and Technology Innovation Project of Hebei Academy of Agricultural and Forestry Sciences 2024KJCXZX-LYS-15Shandong Province Key R&D Plan 2024LZGCQY012Taishan Scholar Foundation of Shandong Province TSCY202211144
6 · The paper itself

Abstract

backgroundCommon wheat (Triticum aestivum L.) is a vital source of nutrition for human consumption. However, wheat production is significantly threatened by various diseases, such as powdery mildew, a widespread fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). Utilizing and identifying resistance genes and elucidating the molecular mechanisms underlying this resistance are the most effective and sustainable ways to fight this disease.

resultsLxd-682, a cultivated emmer wheat accession, exhibited resistance to 12 out of 13 tested Bgt isolates at the seedling stage. Genetic analysis revealed that this resistance is conferred by a single dominant gene, tentatively designated as PmLxd-682. Molecular mapping positioned PmLxd-682 between the markers WGRE77413 and WGRC1096, with the Pm4-diagnostic marker JS717/JS718 co-segregating. Homology-based cloning and sequence alignment further confirmed that PmLxd-682 is identical to Pm4a. qRT-PCR analysis showed that the alternative splicing PmLxd-682-V2 exhibited higher expression level than that of PmLxd-682-V1 post-Bgt invasion, suggesting its prominent role in fighting Bgt invasion. Additionally, four pathogenesis-related (PR) genes were significantly up-regulated in both Lxd-682 and susceptible parent Langdon upon infection, revealing possibly unimportant roles in resistance pathway. Furthermore, 1,567 differentially expressed genes (DEGs) between resistant and susceptible bulks were identified through BSR-Seq, with 490 ones located within the candidate interval on chromosome 2AL, and potential biological processes associated with resistance were enriched via gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. To verify the potential regulatory genes, three key genes, TRITD2 Av1G294940, TRITD2 Av1G036490 and TRITD2 Av1G295220 all encoding disease resistance protein, were selected from six candidates via qRT-PCR following post-Bgt invasion. Molecular markers JS717/JS718 and WGRC1096 were confirmed to be available for marker-assisted selection (MAS) of PmLxd-682 in breeding practices.

conclusionsThe study identified key genetic intervals and genes involved in the resistance of a cultivated emmer wheat accession Lxd-682 to powdery mildew. These findings significantly advance our understanding of plant-pathogen interactions and establish a solid foundation for future genetic and functional studies aimed at improving disease resistance in crops.

Indexed as

AscomycotaDisease ResistancePlant DiseasesTriticumChromosome MappingGenes, PlantSequence Analysis, RNACultivated emmer wheatGene mappingHomologous cloningMASPowdery mildew

Identifiers

PMID40319256
PMCPMC12048989

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