Functional analysis of the woody oil crop Plukenetia volubilis L. LEC2 homolog PvoB3-69 in promoting regeneration.

Jing-Jing Yu, Shiling Deng, Jinhui Mo, Han Huang, Zeng-Fu Xu, Yi Wang

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Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Jing-Jing Yu *Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast‑Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, 530000, China.

Shiling Deng *Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast‑Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, 530000, China.

Jinhui MoKey Laboratory of National Forestry and Grassland Administration on Cultivation of Fast‑Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, 530000, China.

Han HuangKey Laboratory of National Forestry and Grassland Administration on Cultivation of Fast‑Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, 530000, China.

Zeng-Fu XuKey Laboratory of National Forestry and Grassland Administration on Cultivation of Fast‑Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, 530000, China. zfxu@gxu.edu.cn.

Yi WangKey Laboratory of National Forestry and Grassland Administration on Cultivation of Fast‑Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, 530000, China. wangyi1984@gxu.edu.cn.ORCID http://orcid.org/0009-0005-9846-3066

Funding

National Natural Science Foundation of China 32360398Natural Science Foundation of Guangxi Zhuang Autonomous Region 2021JJA130403Specific Research Project of Guangxi for Research Bases and Talents AD23026337

6 · The paper itself

Abstract

key messageIdentified a robust regeneration-related genomic sequence of PvoB3-69 from the B3 superfamily in Plukenetia volubilis. gPvoB3-69-assisted transformation of P. volubilis obtained transgenic shoots for the first time. The regenerative capacity of host cells is critical for the genetic transformation efficiency of woody plants. The B3 superfamily is particularly involved in early embryo morphogenesis and late-stage embryo maturation. In this study, 74 PvoB3 members were identified in the genome of P. volubilis, and classified into four subfamilies: LAV, RAV, ARF, and REM. RNA-seq and RT-qPCR analyses revealed that PvoB3-69, a member of the LAV subfamily, has specific expression patterns similar with LEC2. Overexpression of gPvoB3-69 enhanced the bud regeneration capacity of transgenic Nicotiana benthamiana organs during in vitro culture. Additionally, overexpression of gPvoB3-69 significantly improved somatic embryogenesis in transgenic Arabidopsis plants, especially with the aid of 2,4-D. Transcriptome analysis in Arabidopsis thaliana revealed that PvoB3-69 may enhance somatic embryo induction efficiency by activating developmental regulators such as WUS and LEC1, while also modulating salicylic acid, ABA, and ethylene metabolism. Furthermore, overexpression of gPvoB3-69 in the cotyledons of P. volubilis increased the regeneration ability of host cells, and broke the genetic transformation barrier through the Agrobacterium-mediated method, allowing the regenerated transgenic shoots obtained for the first time. This study provides the first systematic analysis of the B3 superfamily in P. volubilis, identifying PvoB3-69 as a key regulator of regeneration. These findings establish a foundation for further comprehensive studies of PvoB3 genes and deepen our understanding of the regulatory mechanism of shoot regeneration in P. volubilis.

Indexed as

LamialesPlant ProteinsRegenerationArabidopsisGene Expression Regulation, PlantNicotianaPhylogenyPlants, Genetically ModifiedPlant ProteinsB3 superfamilyPlant regenerationPlukenetia volubilisPvoB3-69Transgenic shoots

Identifiers

PMID40319197

DOI10.1007/s00299-025-03493-y

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