Evidence map›Paper›PMID 40316745›Full record

ArticleTransgenic research2025

Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation.

Yanhui Yang, Jun Lu, Tianrui Xue, Zhenli Cao, Mingjie Li, Zhongyi Zhang, Rong Wang, Tongyu Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. IMAGE: INTEGRATE-MediatedFrontiers in microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yanhui YangSchool of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zone, Zhengzhou, 450001, Henan Province, China. yyhui2004@haut.edu.cn.
Jun LuSchool of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zone, Zhengzhou, 450001, Henan Province, China.
Tianrui XueSchool of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zone, Zhengzhou, 450001, Henan Province, China.
Zhenli CaoSchool of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zone, Zhengzhou, 450001, Henan Province, China.
Mingjie LiCollege of Crop Sciences, Fujian Agriculture and Forestry University, Jinshan Road, Cangshan District, Fuzhou, 350002, China.
Zhongyi ZhangCollege of Crop Sciences, Fujian Agriculture and Forestry University, Jinshan Road, Cangshan District, Fuzhou, 350002, China.
Rong WangSchool of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zone, Zhengzhou, 450001, Henan Province, China.
Tongyu WuSchool of Bioengineering, Henan University of Technology, Lianhua Street 100, Zhengzhou High-Technology Zone, Zhengzhou, 450001, Henan Province, China.

Funding

Key Research and Development and Promotion Special Project of Henan Province 242102311169National Natural Science Foundation of China 82274049National Natural Science Foundation of China 82373994
6 · The paper itself

Abstract

The Agrobacterium tumefaciens mediated transformation is the prevailing methodology for plant genetic manipulation; however, A. tumefaciens overgrowth is a common constraint in the process. Exploring auxotrophic A. tumefaciens could reduce overgrowth and enhance plant transformation efficiency. The ILVC gene, which encodes the ketol-acid isomeroreductase, is critical for Valine (Val) and isoleucine (Ile) biosynthesis in some microorganisms. However, its function in A. tumefaciens is unclear. To ascertain the function of ILVC and generate an auxotrophic A. tumefaciens, this study employed an allelic exchange to disrupt the ILVC in A. tumefaciens strain GV3101. This resulted in the loss of ketol-acid isomeroreductase activity and the prevention of Val and Ile biosynthesis, creating a dual-auxotrophic GV3101

Indexed as

Agrobacterium tumefaciensBacterial ProteinsIsoleucinePlants, Genetically ModifiedValineGene Knockout TechniquesNicotianaTransformation, GeneticBacterial ProteinsIsoleucineValineAgrobacterium tumefaciensAuxotrophyILVC genePlant transformationValine and isoleucine biosynthesis

Identifiers

PMID40316745

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.