ArticleScientific reports2025
Proteomic approach for evaluating cryoprotectant formulations for enhanced post-cryopreservation recovery of yeast.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Optimizing cryopreservation protocols of Saccharomyces eubayanus using heat transfer modeling.Applied microbiology and biotechnology · 2026Article
- In-Cell Proteomics Enables High-Resolution Temporal Profiling of Cell Cycle Progression and DNA Damage Response in Saccharomyces cerevisiae.Proteomics · 2026Article
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Authors and funding
6 authors.
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Abstract
Fungi have numerous potential biotechnological applications across the life sciences. To ensure these microorganisms are available for future research, it is essential that they are properly preserved to safeguard against genetic changes, cellular instability, and loss of viability. While the use of cryoprotective agents (CPA) is critical for increasing survival of the material during preservation, the wide adoption of glycerol and DMSO as CPAs may not always be ideal as fungal diversity and functionality are ever growing. Therefore, in the following work, we focused on developing robust cryopreservation formulations that can efficiently preserve fungal strains while also maximizing recovery. Here, 10 different cryopreservation formulations consisting of individual or a combination of CPAs were evaluated for their effect on the Saccharomyces cerevisiae (ATCC 7754) proteome. Spot assays were performed to study the recovery response of each formulation. Functional proteomic and KEGG pathway analyses were used to investigate the molecular mechanism of cold-stress response in S. cerevisiae. A total of 2,299 proteins were identified; depending on the formulation used, a range of 116-1,241 proteins were found to be significantly upregulated and downregulated, indicating the influence of individual formulations. To the best of our knowledge, this is the first study that uses a proteomic-based approach to investigate how different cryopreservation formulations affect key mechanisms within a model organism.
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