ArticleNature communications2025
Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level.
Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Graft Biology in the CRISPR Era: From Tissue Fusion to Genome Compatibility.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits.Advanced biotechnology · 2026Review
- Calcium signaling in crops.The New phytologist · 2026Review
- Beyond efficiency: The multi-scale architecture of robust water transport in plants.Quantitative plant biology · 2026Review
- Beyond GMOs: transgene-free gene-edited crops for global food security.Frontiers in plant science · 2026Review
- Protocol for triggering plant gene silencing by viral delivery of short RNA.STAR protocols · 2025Article
- A CRISPR-Cas9 library to target putative redundant gene sets facilitates their functional exploration in grain development in rice.BMC plant biology · 2025Article
- Climate Change Impacts on Greenhouse Horticulture in the Mediterranean Basin: Challenges and Adaptation Strategies.Plants (Basel, Switzerland) · 2025Review
- Comparative genomics-driven design of virus-delivered short RNA inserts triggering robust gene silencing.Plant biotechnology journal · 2025Article
- Genome-Wide Analysis of Callose Synthase (CALS) Genes in Cabbage (International journal of molecular sciences · 2025Article
- Optimized Auxin and Cytokinin Interactions Enable Direct Somatic Embryogenesis in the Peach Rootstock 'GuardianInternational journal of molecular sciences · 2025Article
- Thermotolerance for yield and quality in rice: a novel NF-Ys-QT12 gene system.Functional & integrative genomics · 2025Article
- CRISPR activation: identifying and using novel genes for plant disease resistance breeding.Frontiers in genome editing · 2025Review
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Genetic variance is vital for breeding programs and mutant screening, yet traditional mutagenesis methods wrestle with genetic redundancy and a lack of specificity in gene targeting. CRISPR-Cas9 offers precise, site-specific gene editing, but its application in crop improvement has been limited by scalability challenges. In this study, we develop genome-wide multi-targeted CRISPR libraries in tomato, enhancing the scalability of CRISPR gene editing in crops and addressing the challenges of redundancy while maintaining its precision. We design 15,804 unique single guide RNAs (sgRNAs), each targeting multiple genes within the same gene families. These sgRNAs are classified into 10 sub-libraries based on gene function. We generate approximately 1300 independent CRISPR lines and successfully identify mutants with distinct phenotypes related to fruit development, fruit flavor, nutrient uptake, and pathogen response. Additionally, we develop CRISPR-GuideMap, a double-barcode tagging system to enable large-scale sgRNA tracking in generated plants. Our results demonstrate that multi-targeted CRISPR libraries are scalable and effective for large-scale gene editing and offer an approach to overcome gene functional redundancy in basic plant research and crop breeding.
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