Evidence map›Paper›PMID 40316209›Full record

ArticleJournal of advanced research2025

Suppressing wheat sucrose phosphate synthase 1-B protects wheat against stripe rust.

Yan Yan, Meng-Lu Wang, Zhen Zhang, Guoyu Liu, Wen-Xin Wei, Xin-Tian Shi, Chen Lan, Xuebin Zhang, Shengchun Xu, Faheem Shehzad Baloch and 4 more

Abstract read
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In one paragraph

Article in Journal of advanced research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yan YanFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China; Xianghu Laboratory, Hangzhou 311231, China; School of Life Sciences, Fudan University, Shanghai 200438, China.
Meng-Lu WangFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Zhen ZhangFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Guoyu LiuFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Wen-Xin WeiFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Xin-Tian ShiFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Chen LanState Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Jinming Road, Kaifeng 475004, China.
Xuebin ZhangState Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Jinming Road, Kaifeng 475004, China.
Shengchun XuXianghu Laboratory, Hangzhou 311231, China.
Faheem Shehzad BalochDepartment of Biotechnology, Faculty of Science, Mersin University, Yenişehir, Mersin 33343, Turkey; Department of Plant Resources and Environment, Jeju National University, Jeju 63243, Republic of Korea.
Awais RasheedDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Zhongfu NiFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Qixin SunFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China.
Jin-Ying GouFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing 100193, China. Electronic address: jygou@cau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionStripe rust caused by Puccinia striiformis Westend. f. sp. tritici (Pst) is a highly destructive wheat disease that threatens global food security. Pst extracts energy from wheat by interfering with photosynthesis, leading to significant yield losses. Redirecting metabolite flux to counteract pathogens remains a major challenge in enhancing crop resilience.

objectiveThe primary objective of this study is to clarify the regulations of sucrose synthesis in wheat during its interaction with Pst, especially in relation to susceptibility and resistance response, and to supply genetic resources for breeding programs dedicated to ensuring food security.

methodsUtilizing bulked segregant RNA sequencing (BSR-Seq), we identified and cloned a novel susceptibility (S) gene, sucrose 6 - phosphate synthase 1 (SPS1). We investigated the transcriptional and post-translational regulations of SPS1 by Pst, the wheat APETALA2 transcription factor (wAP2), and Wheat Kinase START 1 (WKS1, Yr36) in transgenic plants using molecular and biochemical approaches. Sugar content variations were analyzed using gas chromatography-mass spectrometry (GC/MS) and colorimetric assays, while Pst infection dynamics were examined by staining or quantifying biomass and uredinial pustule densities.

resultsTargeted mutagenesis of the Pst-inducible SPS1-B gene significantly reduced sucrose content accumulation and restricted Pst growth without compromising yield. In contrast, over-expressing SPS1-B enhanced Pst growth, confirming its role as a susceptibility (S) gene to Pst. Pst upregulated SPS1-B under optimal conditions, enhancing its own pathogenic success. Conversely, wAP2 suppressed SPS1-B transcription, reduced SPS1 protein level, and inhibited Pst infection intensity in transgenic wheat lines. Moreover, WKS1, a high-temperature adult-plant resistance protein, bound, phosphorylated, and suppressed SPS1-B at the post-translational level.

conclusionThis study identifies SPS1-B as a pivotal molecular switch in sucrose metabolism hijacked by Pst to support its infection. The characterization of SPS1-B and its upstream regulators provides multiple genetic targets for enhancing wheat resistance against stripe rust.

Indexed as

Disease ResistanceGlucosyltransferasesPlant DiseasesPlant ProteinsPucciniaTriticumBasidiomycotaGene Expression Regulation, PlantPlants, Genetically ModifiedSucroseGlucosyltransferasesPlant ProteinsSucrosesucrose-phosphate synthaseAPETALA2 transcription factorStripe rustSucrose-phosphate synthaseSusceptibility geneWheat

Identifiers

PMID40316209
PMCPMC12302701

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.