Evidence map›Paper›PMID 40315381›Full record

ArticleAnalytical chemistry2025

Leukocyte Depletion in Dried Blood Spot Cards Enables Enrichment of Parasite DNA for Improved Sequencing.

Allison J Tierney, Surendra K Prajapati, Alec Leonetti, Abebe A Fola, Sebastian Shine Kwapong, Keith R Baillargeon, Ashleigh Roberds, V Ann Stewart, Linda E Amoah, Jeffrey A Bailey and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Allison J TierneyDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Surendra K PrajapatiDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, United States.
Alec LeonettiDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island 02903, United States.
Abebe A FolaDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island 02903, United States.
Sebastian Shine KwapongImmunology Department, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra GA-337-3045, Ghana.
Keith R BaillargeonDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.
Ashleigh RoberdsDepartment of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, United States.
V Ann StewartDepartment of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, United States.
Linda E AmoahImmunology Department, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra GA-337-3045, Ghana.
Jeffrey A BaileyDepartment of Pathology and Laboratory Medicine, Warren Alpert Medical School, Brown University, Providence, Rhode Island 02903, United States.
Kim C WilliamsonDepartment of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, United States.ORCID 0000-0003-2749-4082
Charles R MaceDepartment of Chemistry, Tufts University, Medford, Massachusetts 02155, United States.ORCID 0000-0003-3410-5014

Funding

Tracking the flow of malaria parasites and drug resistance within the DRC and across its bordersR01AI139520 · NIAID · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI BAILEY, JEFFREY A. · 2018 to 2023
$3.6M
Artemisinin Resistance in Africa: its emergence and evolution in RwandaR01AI156267 · NIAID · BROWN UNIVERSITY · PI BAILEY, JEFFREY A., JULIANO, JONATHAN J · 2021 to 2024
$3.0M
Precision metering and autonomous separation of blood components for pre-clinical microsampling at the point-of-careR01EB035549 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI Charles R Mace · 2025 to 2026
$1.1M
NIAID NIH HHS R01 AI139520NIAID NIH HHS R01 AI156267NIBIB NIH HHS R01 EB035549
6 · The paper itself

Abstract

Expanding access to simple blood collection tools is essential to monitor, control, and eliminate malaria in low-resource settings where the disease is endemic. The most common method to preserve blood is depositing fingerstick samples onto filter paper─the dried blood spot (DBS) card. While DBS cards offer more optimal storage solutions than venous blood in vacutainers, they do not provide sample cleanup or enrichment of

Indexed as

DNA, ProtozoanDried Blood Spot TestingLeukocyte Reduction ProceduresLeukocytesMalaria, FalciparumPlasmodium falciparumHumansSequence Analysis, DNADNA, Protozoan

Identifiers

PMID40315381
PMCPMC12724477

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.