ArticleAnalytical chemistry2025
Accurate Determination of Circulatory Lipids Using a Combination of HILIC-MRM and RPLC-PRM.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Fast untargeted and targeted high-resolution mass spectrometry workflows for large-scale lipidomics.Analytica chimica acta · 2026Article
- Divergent lipid utilization strategies of SARS-CoV-2 and MERS-CoV revealed by comparative multi-omics profiling of infected mouse lung tissues.Frontiers in immunology · 2026Article
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Authors and funding
4 authors.
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Abstract
Circulatory lipids are important markers for characterizing disease phenotypes; however, accurately determining lipid species remains a significant challenge in lipidomic analysis. Here, we present a novel analytical workflow for accurate lipidome characterization in human plasma using mass spectrometry (MS) through the integration of hydrophilic interaction liquid chromatography (HILIC) and reversed-phase liquid chromatography (RPLC). This workflow enables rapid screening of 1,966 lipid species across 18 lipid classes using HILIC-multiple reaction monitoring (MRM), which enables facile identification of lipid species by lipid class-based separations. In the NIST Standard Reference Material for Human Plasma (SRM 1950), 489 lipid species were identified using HILIC-MRM and subsequently analyzed with RPLC-parallel reaction monitoring (PRM) to resolve potential lipid isobars within the same lipid class. Notably, RPLC-PRM identified 70 additional lipidomic features in SRM 1950 that were not detectable with HILIC-MRM. Furthermore, a high correlation (Pearson correlation coefficient = 0.81) was observed regarding the concentrations of lipid species not carrying isobaric interferences in between HILIC-MRM and RPLC-PRM, indicating that the individual lipid concentrations measured by each platform can be integrated. The workflow was further applied to a cohort of 284 human plasma samples from chronic kidney disease (CKD) patients, successfully profiling lipidomic phenotypes across CKD subtypes. These findings demonstrate that combining HILIC-MRM and RPLC-PRM as complementary platforms enhances the accuracy and comprehensiveness of lipidomic analysis.
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