Evidence map›Paper›PMID 40314193›Full record

ArticlePharmaceutical biology2025

Delivery of small interfering RNA through lyophilized natural lipid nanoparticles: effects of natural lipid selection.

Hangjie Wang, Wei Li, Junyan Chen, Rong Chen, Yuwei Qi, Linshuang Shen, Kaidi Chen, Lewei Dai, Yuxin Sheng, An Wang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Hangjie WangSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Wei LiAcademy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Junyan ChenSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Rong ChenSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Yuwei QiSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Linshuang ShenSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Kaidi ChenSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Lewei DaiSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Yuxin ShengSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
An WangSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Hong WangSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.
Chujian ChenHuzhou Institute for Food and Drug Control, Huzhou, P. R. China.
Xiao ChengHuzhou Institute for Food and Drug Control, Huzhou, P. R. China.
Mancang GuSchool of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, P. R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

contextLipid nanoparticles (LNPs) are the primary non-viral vectors for siRNA delivery. However, synthetic lipids face issues, such as low lysosomal escape efficiency and high cost.

objectiveThis study aimed to use three natural lipids to construct LNPs, optimize their preparation and freeze-drying processes, and evaluate their siRNA delivery efficiency MATERIALS AND

methods

resultsThe optimal LNPs exhibited a size of 160-180 nm, zeta of 44-50 mV, and PDI of <0.2. At 200 μg/mL, the LNPs did not affect cell viability. CSL-LNPs, BJL-LNPs, and SO-LNPs reduced KRAS DISCUSSION AND

conclusionNatural lipids are promising components for LNPs construction, offering new avenues for siRNA delivery in gene therapy.

Indexed as

LipidsNanoparticlesRNA, Small InterferingCell SurvivalFreeze DryingHumansLiposomesParticle SizeSeedsLipid NanoparticlesLipidsLiposomesRNA, Small InterferingBrucea javanica seed lipidCoix seed lipidcryodesiccationendosomal escapelipid droplet targetingsiRNA transfectionsoybean oil

Identifiers

PMID40314193
PMCPMC12051533

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.