Evidence map›Paper›PMID 40314062›Full record

ArticleJournal of extracellular vesicles2025

Beta-Lactam Antibiotics Promote Extracellular Vesicle Production of Staphylococcus aureus Through ROS-Mediated Lipid Metabolic Reprogramming.

Xiaonan Huang, Zhen Hu, Weilong Shang, Juan Chen, Qiwen Hu, Yumin Zhou, Ruolan Ding, Jing Yin, Mengyang Li, He Liu and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Towards a Scalable Production ofInternational journal of molecular sciences · 2026
    Article
  4. Review
  5. Review
  6. Homotypic Membrane Vesicle-Formulated VAN@Biomaterials research · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Xiaonan HuangDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Zhen HuDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Weilong ShangDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Juan ChenDepartment of Pharmacy, Xinqiao Hospital, Army Medical University, Chongqing, China.
Qiwen HuDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Yumin ZhouDepartment of Dermatology, Southwest Hospital, Army Medical University, Chongqing, China.
Ruolan DingDepartment of Microbiology, School of Medicine, Chongqing University, Chongqing, China.
Jing YinDepartment of Neurology, First Affiliated Hospital of Kunming Medical University, Kunming, China.
Mengyang LiDepartment of Microbiology, School of Medicine, Chongqing University, Chongqing, China.
He LiuDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Jianxiong DouDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Huagang PengDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Yifan RaoDepartment of Emergency Medicine, Xinqiao Hospital, Army Medical University, Chongqing, China.
Lu LiuDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Yuting WangDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Li TanDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Yuhua YangDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Jianghong WuDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Chuan XiaoDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Yi YangDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.
Xiancai RaoDepartment of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Army Medical University, Chongqing, China.ORCID https://orcid.org/0000-0002-9905-760X

Funding

National Natural Science Foundation of China 82071857
6 · The paper itself

Abstract

Bacterial extracellular vesicles (EVs) are natural reservoirs of biological active substances. They exhibit promising application in developing bioproducts such as vaccine, drug-delivery system and anticancer agent. However, the low yield of naturally secreted EVs during bacterial growth is a bottleneck factor that restricts EV applications. In this study, we showed that sub-minimum inhibitory concentration (MIC) of β-lactams boosted EV production in various Staphylococcus aureus strains. The expression of penicillin-binding protein (PBP) genes increased after β-lactam treatment, and the inactivation of alternative PBPs promoted EV secretion of S. aureus. We also demonstrated that sub-MIC β-lactams promoted EV production via a reactive oxygen species (ROS)-dependent pathway. Deletion of redundant pbp genes enhanced oxacillin (OXA)-stimulated ROS levels. Transcriptomic and lipidomic analyses revealed that OXA-induced ROS triggered lipid metabolic reprogramming in S. aureus. Particularly, ROS promoted lipid peroxidation (LPO) and increased the biosynthesis of phosphatidic acid (PA) and lipoteichoic acid (LTA) that contributed to EV generation. Furthermore, OXA treatment altered the diversity of EV-loaded proteins. OXA-treated

Indexed as

Anti-Bacterial Agentsbeta-LactamsExtracellular VesiclesLipid MetabolismReactive Oxygen SpeciesStaphylococcus aureusAnimalsbeta Lactam AntibioticsMetabolic ReprogrammingMiceMice, Inbred BALB CMicrobial Sensitivity TestsOxacillinStaphylococcal InfectionsAnti-Bacterial Agentsbeta Lactam Antibioticsbeta-LactamsOxacillinReactive Oxygen Speciesextracellular vesiclelipid metabolismpenicillin binding proteinsreactive oxygen speciesStaphylococcus aureusβ‐lactam antibiotics

Identifiers

PMID40314062
PMCPMC12046293

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.