Evidence map›Paper›PMID 40313918›Full record

ArticlebioRxiv : the preprint server for biology2025

Nucleic acid sequence determinants of transcriptional pausing by human mitochondrial RNA polymerase (POLRMT).

An H Hsieh, Tatiana V Mishanina

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

An H HsiehDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA.ORCID 0000-0003-3552-1132
Tatiana V MishaninaDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA.

Funding

MOLECULAR BIOPHYSICS TRAINING PROGRAMT32GM008326 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI KOMIVES, ELIZABETH A. · 1989 to 2020
$7.5M
Mechanisms of transcription and co-transcriptional gene expression processes in bacteria and human mitochondriaR35GM142785 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Tatiana Vladimirovna Mishanina · 2021 to 2026
$2.5M
NIGMS NIH HHS R35 GM142785NIGMS NIH HHS T32 GM008326
6 · The paper itself

Abstract

Transcription by RNA polymerase (RNAP) lies at the heart of gene expression in all organisms. The speed with which RNAPs produce the RNA is tuned in part by the signals in the transcribed nucleic-acid sequences, which temporarily arrange RNAPs into a paused conformation unable to extend the RNA. In turn, the altered transcription kinetics determines the three-dimensional shape into which RNA ultimately folds, dictates the chromatin state, and promotes or inhibits co-transcriptional events. While pause sequence determinants have been characterized for multi-subunit RNAPs in bacteria and the eukaryotic nuclei, this information is lacking for the single-subunit RNAP of human mitochondria, POLRMT. Here, we developed a robust nucleic-acid scaffold system to reconstitute POLRMT transcription

Identifiers

PMID40313918
PMCPMC12045343

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.