Evidence map›Paper›PMID 40313752›Full record

ArticleResearch square2025

Precise calcium-to-spike inference using biophysical generative models.

Gerard Joey Broussard, Giovanni Diana, Francisco J Urra Quiroz, B Semihcan Sermet, Nelson Rebola, Laura A Lynch, David A DiGregorio, Samuel S-H Wang

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Gerard Joey BroussardNeuroscience Institute, Washington Road, Princeton University, Princeton, New Jersey USA 08540.
Giovanni DianaInstitut Pasteur, Université Paris Cité, CNRS UMR 3571, Synapse and Circuit Dynamics Laboratory, Paris, France.
Francisco J Urra QuirozInstitut Pasteur, Université Paris Cité, CNRS UMR 3571, Synapse and Circuit Dynamics Laboratory, Paris, France.
B Semihcan SermetInstitut Pasteur, Université Paris Cité, CNRS UMR 3571, Synapse and Circuit Dynamics Laboratory, Paris, France.ORCID 0000-0002-9983-3605
Nelson RebolaL'Institut du Cerveau et de la Moelle épinière, CNRS UMR 7225, Paris, France.
Laura A LynchNeuroscience Institute, Washington Road, Princeton University, Princeton, New Jersey USA 08540.
David A DiGregorioInstitut Pasteur, Université Paris Cité, CNRS UMR 3571, Synapse and Circuit Dynamics Laboratory, Paris, France.
Samuel S-H WangNeuroscience Institute, Washington Road, Princeton University, Princeton, New Jersey USA 08540.ORCID 0000-0002-0490-9786

Funding

Subcortical Neural Coding and DynamicsU19NS104648 · NINDS · PRINCETON UNIVERSITY · PI BRODY, CARLOS D, GOLDMAN, MARK S · 2017 to 2021
$15.3M
Imaging adaptive cerebellar processing at cellular resolution in awake miceR01NS045193 · NINDS · PRINCETON UNIVERSITY · PI WANG, SAMUEL SHENG-HUNG · 2003 to 2024
$7.5M
Use of Calcium Indicator Proteins in Spike Counting ModeR21EY026434 · NEI · PRINCETON UNIVERSITY · PI DIGREGORIO, DAVID A, WANG, SAMUEL SHENG-HUNG · 2015 to 2016
$476k
NEI NIH HHS R21 EY026434NINDS NIH HHS R01 NS045193NINDS NIH HHS U19 NS104648
6 · The paper itself

Abstract

The intramolecular dynamics of fluorescent indicators of neural activity can distort the accurate estimate of action potential ("spike") times. In order to develop a more accurate spike inference algorithm we characterized the kinetic responses to calcium of three popular indicator proteins, GCaMP6f, jGCaMP7f, and jGCaMP8f, using in vitro stopped-flow and brain slice recordings. jGCaMP8f showed a use-dependent slowing of fluorescence responses that caused existing inference methods to generate numerous false positives. From these data we developed a multistate model of GCaMP and used it to create Bayesian Sequential Monte Carlo (Biophys

Identifiers

PMID40313752
PMCPMC12045362

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.