Evidence map›Paper›PMID 40313010›Full record

ArticleFEBS letters2025

Cytoplasmic delivery of antibodies through grafting a functional single complementarity-determining region loop.

Yerin Jeon, Juho Choi, Youngin Roh, Eunbin Lee, Luigie Villamante, Myung-Hee Kwon

Abstract read
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In one paragraph

Article in FEBS letters, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yerin JeonDepartment of Biomedical Sciences, Graduate School, Ajou University, Suwon, South Korea.
Juho ChoiDepartment of Microbiology, Ajou University School of Medicine, Suwon, South Korea.
Youngin RohDepartment of Biomedical Sciences, Graduate School, Ajou University, Suwon, South Korea.
Eunbin LeeDepartment of Biomedical Sciences, Graduate School, Ajou University, Suwon, South Korea.
Luigie VillamanteDepartment of Biomedical Sciences, Graduate School, Ajou University, Suwon, South Korea.
Myung-Hee KwonDepartment of Biomedical Sciences, Graduate School, Ajou University, Suwon, South Korea.ORCID https://orcid.org/0000-0001-8757-3693

Funding

National Research Foundation of Korea 22020R1A2C2008258
6 · The paper itself

Abstract

The mouse 3D8 anti-DNA antibody can enter cells and localize in the cytoplasm, primarily facilitated by the complementarity-determining region 1 of the variable light chain (CDR L1) domain. In this study, we grafted the CDR L1 loop from 3D8 onto non-cell-penetrating IgG antibodies to investigate whether these IgGs could acquire cytoplasmic localization ability while retaining antigen-binding activity. One of three IgGs was successfully delivered into the cytoplasm while maintaining antigen-binding activity. In silico protein modeling suggests that this capability is linked to structural similarity between CDR L1 in the grafted Ab and that in 3D8. This study proposes a strategy to confer cell-penetrating capability by incorporating a specific CDR loop into an antibody backbone while retaining affinity.

Indexed as

Antibodies, AntinuclearComplementarity Determining RegionsCytoplasmImmunoglobulin GAmino Acid SequenceAnimalsHumansMiceModels, MolecularAntibodies, AntinuclearComplementarity Determining RegionsImmunoglobulin Ganti‐DNA antibodyCDR graftingcell penetrationcytoplasmic deliveryIgG

Identifiers

PMID40313010
PMCPMC12104855

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.