ArticleNature methods2025
ATLAS: a rationally designed anterograde transsynaptic tracer.
Article in Nature methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- A ventral hippocampus to nucleus accumbens pathway regulates impulsivity in male but not female rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping.Research square · 2026Article
- Transsynaptic neural circuit mapping of ventral hippocampus motivational control systems.Brain structure & function · 2026Article
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- Tracer Techniques in Ophthalmology: Ocular Applications and Systemic Connections.Diagnostics (Basel, Switzerland) · 2026Review
- The role of transthalamic pathways in perception.Communications biology · 2026Review
- BrainConnect: processing brain connectivity and spatial transcriptomics data for integrative analysis.Bioinformatics (Oxford, England) · 2026Article
- Rostral Ventromedial Medulla (RVM) Projects to the Lateral Hypothalamic Area (LHA) to Drive Aversion and Anxiety.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Distinct roles of prefrontal subregion feedback to the primary visual cortex across behavioral states.Neuron · 2026Article
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- A ventral hippocampus to nucleus accumbens pathway regulates impulsivity.bioRxiv : the preprint server for biology · 2025Article
- The synaptic vesicle cluster as a controller of pre- and postsynaptic structure and function.The Journal of physiology · 2025Review
- Prefrontal Cortex subregions provide distinct visual and behavioral feedback modulation to the Primary Visual Cortex.bioRxiv : the preprint server for biology · 2025Article
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18 authors.
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Abstract
Genetically modified rabies virus can map neural circuits retrogradely from genetically determined cells. However, similar tools for anterograde tracing are not available. Here, we describe a method for anterograde transsynaptic tracing from genetically determined neurons based on a rationally designed protein, ATLAS. Expression of ATLAS in neurons causes presynaptic release of a payload composed of an antibody-like protein, AMPA.FingR, which binds to the N terminus of GluA1, and a recombinase. In the synaptic cleft, AMPA.FingR binds to GluA1, causing the payload to be endocytosed into postsynaptic cells and delivered to the nucleus, where it triggers expression of a recombinase-dependent reporter. In mice, ATLAS mediates monosynaptic transneuronal tracing from random or genetically determined cells that is strictly anterograde, synaptic and nontoxic. Moreover, ATLAS-mediated tracing shows activity dependence, suggesting that it can label active circuits underlying specific behaviors. Finally, ATLAS is composed of modular components that can be independently replaced or modified.
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