Evidence map›Paper›PMID 40311753›Full record

ArticleJournal of advanced research2026

Proteomic profiling in cerebrospinal fluid reveal biomarkers for shunt outcome in idiopathic normal-pressure hydrocephalus.

Yuqi Ying, Jingquan Lin, Wei Gao, Liang Yue, Qingze Zeng, Katrina Bartas, Dayeon Cheong, Hongjie Jiang, Zhe Zheng, Ligen Shi and 9 more

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Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 2 of them syntheses that pooled it.

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4citing papers in PubMed, 2 pooled it
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1 · What the graph read from it

What it found

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3 · Its place in the literature

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4 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 · The record

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5 · Who and what money

Authors and funding

19 authors.

Yuqi YingDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Department of Neurosurgery, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu 322000, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Jingquan LinDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Wei GaoDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Liang YueAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang Province, China; Research Center for Industries of the Future, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.
Qingze ZengDepartment of Radiology, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Katrina BartasDepartment of Physiology and Biophysics, University of California, Irvine, CA 92697, USA.
Dayeon CheongDepartment of Physiology and Biophysics, University of California, Irvine, CA 92697, USA.
Hongjie JiangDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Zhe ZhengDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Ligen ShiDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
An PingDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Yuanjian FangDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Feng YanDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China.
Tiannan GuoAffiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China; Westlake Center for Intelligent Proteomics, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang Province, China; Research Center for Industries of the Future, School of Life Sciences, Westlake University, Hangzhou, Zhejiang Province, China.
Jianmin ZhangDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Department of Neurosurgery, The Fourth Affiliated Hospital, International Institutes of Medicine, Zhejiang University School of Medicine, Yiwu 322000, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China. Electronic address: zjm135@zju.edu.cn.
Hemmings WuDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China. Electronic address: hemmings@zju.edu.cn.
Kevin BeierDepartment of Physiology and Biophysics, University of California, Irvine, CA 92697, USA; Department of Pharmaceutical Sciences, University of California, Irvine, CA 92617, USA; Department of Biomedical Engineering, University of California, Irvine, CA 92617, USA; Department of Neurobiology and Behavior, University of California, Irvine, CA 92617, USA; Center for the Neurobiology of Learning and Memory, University of California, Irvine, CA 92617, USA; UCI Mind, University of California, Irvine, CA 92617, USA. Electronic address: kbeier@uci.edu.
Junming ZhuDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China. Electronic address: dr.zhujunming@zju.edu.cn.
Zhoule ZhuDepartment of Neurosurgery, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou 310009, China; Clinical Research Center for Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; Key Laboratory of Precise Treatment and Clinical Translational Research of Neurological Diseases of Zhejiang Province, Hangzhou 310009, China; State Key Laboratory of Transvascular Implantation Devices, Hangzhou 310009, China. Electronic address: zhuqianle1231@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe pathophysiology of idiopathic normal pressure hydrocephalus (iNPH) remains unclear, and the treatment strategy remains suboptimal. This study aims to identify biomarkers for shunt prognosis by cerebrospinal fluid (CSF) proteomic profiling.

methodsCSF samples collected from 37 iNPH patients from the discovery cohort and 12 iNPH patients from an independent validation cohort (71.9 ± 6.1 years (mean ± SD)), and 16 age-balanced controls (69.9 ± 7.6 years (mean ± SD)) were collected from September 2020 to December 2023. 53 CSF samples were analyzed using a mass spectrometry-based proteomic workflow. Clinical evaluations were performed on all iNPH patients, and 44 patients underwent ventriculoperitoneal shunting. Postoperative CSF were also collected from 10 iNPH patients who underwent shunting surgery. Bioinformatics, machine learning, and enzyme-linked immunosorbent assay (ELISA) were performed to identify CSF proteome changes related to pathophysiology in iNPH, and screen for biomarkers associated with shunt response.

results39 and 285 proteins significantly increased and decreased in iNPH CSF compared to the control group. Gene ontology analysis revealed that the noticeably increased proteins were mainly associated with myeloid leukocyte migration and extracellular matrix organization, and significantly decreased proteins were primarily associated with axon development and synapse organization. Machine learning identified 6 candidate biomarkers that potentially predicted the response to shunt surgery. Among these, QPCT levels were found to be elevated in non-responders, while RBP4 levels were decreased, and both of these changes were validated through ELISA.

conclusionsOur findings provide support for the hypothesis that the pathophysiology of iNPH is characterized by a state of neuroinflammation, extracellular matrix remodeling, and neurodegeneration, and CSF shunting can reverse such pathological state. Machine learning using preoperative proteomic profiles satisfactorily predicted the clinical outcome of the shunt procedure in iNPH. Future research targeting specific proteins in iNPH may be warranted to better comprehend the disease mechanism and design patient-tailored treatments.

Indexed as

BiomarkersHydrocephalus, Normal PressureProteomeProteomicsVentriculoperitoneal ShuntAgedFemaleHumansMaleMiddle AgedPrognosisTreatment OutcomeBiomarkersProteomeCerebrospinal fluidIdiopathic normal pressure hydrocephalusMachine learningProteomicsShunt surgery response

Identifiers

PMID40311753
PMCPMC12869226

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