ArticleBlood advances2025
Use of the microfluidic impedance red cell assay in sickle cell disease.
Article in Blood advances, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Blood rheology after allogeneic hematopoietic stem cell transplantation or gene therapy in sickle cell disease.Blood advances · 2026Article
- Lights, camera, occlusion! watch sickle cells in real time.Blood advances · 2026Article
- Sickle Cell Disease and Male Infertility: Pathophysiological Mechanisms, Clinical Manifestations, and Fertility Preservation Strategies-A Narrative Review.Life (Basel, Switzerland) · 2026Review
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
abstractIn sickle cell disease (SCD), red blood cells (RBCs) are poorly deformable, even under normoxia (NOI). With deoxygenation, deformability of sickle RBCs is further reduced due to polymerization of hemoglobin S (HbS). Rigid, poorly deformable sickle RBCs block microvasculature, causing ischemia, pain, and organ damage. The microfluidic impedance red cell assay (MIRCA) can mechanically measure RBC deformability, providing occlusion index under NOI or hypoxia (HOI) as readouts. We analyzed RBCs from 68 adult and 34 pediatric patients with SCD using the MIRCA. Higher HOI and NOI values were positively associated with markers of inflammation, hemolysis, RBC density, older age, and severe SCD genotypes (homozygous HbSS or HbS β0-thalassemia. Each 1% higher NOI across individuals was associated with a 6.3% higher incidence of acute complications per year. Individuals with chronic complications in the past year had a 3.1% higher median NOI than those without chronic complications. Individuals on chronic transfusion therapy exhibit a subpopulation of poorly deformable RBCs captured by the MIRCA but not by a commercially available device that also measures RBC deformability, the laser assisted optical rotational cell analyzer (LoRRca). In vitro addition of voxelotor or osivelotor to samples from individuals on chronic transfusion therapy improved the deformability of these endogenous RBCs. Longitudinally collected NOI and HOI values in individuals with HbSS were stable, with a median percent point change of 13.3% and 15.7%, respectively. MIRCA can be used in combination with clinical laboratory tests to monitor RBC deformability as a biomarker of clinical status at routine clinic visits and included in clinical trials of disease-modifying agents.
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