ArticleThe Journal of cell biology1985
Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 244 citations in OpenAlex.
- The Trajectory Statistics of Biological Exploratory Dynamics.bioRxiv : the preprint server for biology · 2026Article
- Biological processes as exploratory dynamics.Biophysical journal · 2026Review
- Article
- A celebration of the 25th anniversary of chromatin-mediated spindle assembly.Molecular biology of the cell · 2022Article
- Kinetochore life histories reveal an Aurora-B-dependent error correction mechanism in anaphase.Developmental cell · 2021Article
- Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis.The EMBO journal · 2019Article
- Spindle Dynamics Model Explains Chromosome Loss Rates in Yeast Polyploid Cells.Frontiers in genetics · 2018Article
- In vitro reconstitution of lateral to end-on conversion of kinetochore-microtubule attachments.Methods in cell biology · 2018Article
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Contributions of Microtubule Dynamic Instability and Rotational Diffusion to Kinetochore Capture.Biophysical journal · 2017Article
- Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I.Scientific reports · 2016Article
- In Vitro Kinetochore Assembly.Methods in molecular biology (Clifton, N.J.) · 2016Article
- CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.Molecular biology of the cell · 2015Article
- Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles.PloS one · 2015Article
- Swinging a sword: how microtubules search for their targets.Systems and synthetic biology · 2014Review
- Pivoting of microtubules around the spindle pole accelerates kinetochore capture.Nature cell biology · 2013Article
- Review
- Tao-1 is a negative regulator of microtubule plus-end growth.Journal of cell science · 2010Article
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp.Methods (San Diego, Calif.) · 2010Review
31 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the interaction of preformed microtubules (MTs) with the kinetochores of isolated chromosomes. This reaction, which we call MT capture, results in MTs becoming tightly bound to the kinetochore, with their ends capped against depolymerization. These observations, combined with MT dynamic instability, suggest a model for spindle morphogenesis. In addition, ATP appears to mobilize dynamic processes at captured MT ends. We used biotin-labeled MT seeds to follow assembly dynamics at the kinetochore. In the presence of ATP and unlabeled tubulin, labeled MT segments translocate away from the kinetochore by polymerization of subunits at the attached end. We have termed this reaction proximal assembly. Further studies demonstrated that translocation could be uncoupled from MT assembly. We suggest that the kinetochore contains an ATPase activity that walks along the MT lattice toward the plus end. This activity may be responsible for the movement of chromosomes away from the pole in prometaphase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.