Evidence map›Paper›PMID 40308901›Full record

ArticleApplications in plant sciences

A comprehensive illustrated protocol for clearing, mounting, and imaging leaf venation networks.

Isabella Niewiadomski, Monica Antonio, Luiza Maria T Aparecido, Mickey Boakye, Sonoma Carlos, Andrea Echevarria, Adrian Fontao, Joseph Mann, Ilaíne Silveira Matos, Norma Salinas and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Isabella NiewiadomskiDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0009-0003-9288-6063
Monica AntonioDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0009-0009-0662-0511
Luiza Maria T AparecidoSchool of Biological Sciences University of Utah Salt Lake City Utah USA.ORCID 0000-0001-8642-6632
Mickey BoakyeDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0000-0002-6557-9928
Sonoma CarlosDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0009-0005-7345-3224
Andrea EchevarriaDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.
Adrian FontaoDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0009-0001-5683-451X
Joseph MannDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0009-0008-3082-9123
Ilaíne Silveira MatosDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0000-0001-5557-5133
Norma SalinasDepartment of Science, Chemistry Section, and Institute for Nature Earth and Energy Pontifical Catholic University of Peru Lima Peru.ORCID 0000-0001-9941-2109
Bradley VuDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0009-0007-4529-6828
Benjamin Wong BlonderDepartment of Environmental Science, Policy, and Management University of California at Berkeley Berkeley California USA.ORCID 0000-0002-5061-2385

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Premise: Leaf venation network architecture can provide insights into plant evolution, ecology, and physiology. Venation networks are typically assessed through histological methods, but existing protocols provide limited guidance on processing large or challenging leaves. Methods and results: We present an illustrated protocol for visualizing whole leaf venation networks, including sample preparation, clearing, staining, mounting, imaging, and archiving steps. The protocol also includes supply lists, troubleshooting procedures, safety considerations, and examples of successful and unsuccessful outcomes. The protocol is suitable for a wide range of leaf sizes and morphologies and has been used with all major plant groups. Conclusion: We provide a workflow for obtaining high-quality mounts and images of venation networks of a wide range of species, using readily available materials.

Indexed as

cleared leafhistologyleaf architectureleaf venation networkvascular network

Identifiers

PMID40308901
PMCPMC12038745

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.