Evidence map›Paper›PMID 40308692›Full record

ArticleFrontiers in veterinary science2025

Regulatory element map of sheep reproductive tissues: functional annotation of tissue-specific strong active enhancers.

Zhu Meng, Mingxing Chu, Hao Yang, Shiwen Zhang, Qiangjun Wang, Jiahong Chen, Chunhuan Ren, Zhangyuan Pan, Zijun Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhu Meng *Anhui Provincial Key Laboratory of Conservation and Germplasm Innovation of Local Livestock, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Mingxing Chu *State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Hao YangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Shiwen ZhangState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Qiangjun WangAnhui Provincial Key Laboratory of Conservation and Germplasm Innovation of Local Livestock, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Jiahong ChenAnhui Provincial Key Laboratory of Conservation and Germplasm Innovation of Local Livestock, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Chunhuan RenAnhui Provincial Key Laboratory of Conservation and Germplasm Innovation of Local Livestock, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Zhangyuan PanState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Zijun ZhangAnhui Provincial Key Laboratory of Conservation and Germplasm Innovation of Local Livestock, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Comprehensive functional annotation of the genome is crucial for elucidating the molecular mechanisms underlying complex traits and diseases. Although functional annotation has been partially completed in sheep, a systematic annotation focused on reproductive tissues remains absent. Methods: In this study, we integrated 60 transcriptomic and epigenomic datasets from five reproductive tissues. Using a multi-omics approach, we predicted 15 distinct chromatin states and conducted thorough functional annotation. Results: We established the first regulatory element atlas for sheep reproductive tissues and examined the roles of these elements in reproductive traits and disease. In total, we annotated 1,680,172 regulatory elements, including 83,980 tissue-specific strong active enhancers (EnhAs). Discussion: Enhancers were identified as critical drivers of tissue-specific functions, operating through sequence-specific transcription factor binding and direct regulation of target genes. Key transcription factors associated with reproductive function included

Indexed as

enhancerregulatory elementreproductive tissuessheeptissue specific

Identifiers

PMID40308692
PMCPMC12040938

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.