ArticleMethods in molecular biology (Clifton, N.J.)2025
Co-cultures of Human-Induced Pluripotent Stem Cell-Derived Neurons, Astrocytes, and Microglia for Modeling Neurodegenerative Diseases.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
1 citing paper in PubMed.
- The pathophysiology of mixed Alzheimer's disease and vascular dementia.Theranostics · 2025Review
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14 authors.
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Abstract
Although progressive neurodegenerative diseases like Alzheimer's disease (AD) have been extensively studied for decades, some underlying mechanisms of pathogenesis remain elusive. In addition, modeling neurodegenerative diseases in vitro has proven to be a challenging task. However, advances in the technique of using human induced pluripotent stem cells (hiPSCs) have enabled the scientific community to study hiPSC-derived neurons, astrocytes, or microglia. Despite this important progress, monocultures of individual cell types may not accurately reflect the complexities of modeling specific pathological mechanisms. Therefore, we present a robust protocol for co-cultivating hiPSC-derived cortical neurons, astrocytes, and microglia in a tri-culture system for the purpose of hypothesis testing and drug screening. This co-cultivation system may allow modeling the effect of astrocyte and/or microglia modulation on neuronal health or intra-neuronal Tau aggregation, a key feature of AD progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.