Evidence map›Paper›PMID 40307634›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

One-Step Differentiation of Human-Induced Pluripotent Stem Cells into Podocytes.

Elisabeth Naderlinger, Cormac Murphy, Elisabeth Feifel, Paul Jennings, Gerhard Gstraunthaler, Anja Wilmes

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Elisabeth NaderlingerDepartment of Chemistry and Pharmaceutical Sciences, Division of Molecular and Computational Toxicology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Cormac MurphyDepartment of Chemistry and Pharmaceutical Sciences, Division of Molecular and Computational Toxicology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Elisabeth FeifelDivision of Physiology, Medical University Innsbruck, Innsbruck, Austria.
Paul JenningsDepartment of Chemistry and Pharmaceutical Sciences, Division of Molecular and Computational Toxicology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Gerhard GstraunthalerDivision of Physiology, Medical University Innsbruck, Innsbruck, Austria.
Anja WilmesDepartment of Chemistry and Pharmaceutical Sciences, Division of Molecular and Computational Toxicology, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands. a.wilmes@vu.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Within the glomerulus, podocytes are highly specialized visceral epithelial cells, that are part of the glomerular filtration barrier. Human podocyte cell culture is rather challenging for primary or immortalized cells, due to the non-proliferative state of the cells. In addition, de-differentiation is often observed rapidly. Hence, iPSC-derived podocytes offer an exciting alternative to culture podocyte-like cells from different donors over a prolonged time. Here we report an improved protocol of a simple and rapid one-step differentiation that drives iPSC into podocyte-like cells in 10 days and offers the possibility to transfer the podocyte-like cells into another plate format once.

Indexed as

Cell Culture TechniquesCell DifferentiationInduced Pluripotent Stem CellsPodocytesCells, CulturedHumansDifferentiationiPSCPodocytesVitronectinWeekend-free

Identifiers

PMID40307634

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.