Evidence map›Paper›PMID 40307421›Full record

ArticleScientific reports2025

A novel method for isolation of tumor infiltrating myeloid-derived suppressor cells from human lung tumor tissue.

Marta Piqueras-Nebot, Marta Benet, Miriam Estors, Antonio Cremades, Óscar Juan-Vidal, Julián Carretero, José Marcelo Galbis-Caravajal, Agustín Lahoz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Marta Piqueras-Nebot *Biomarkers and Precision Medicine Unit, Instituto de Investigación Sanitaria La Fe, 46026, Valencia, Spain.
Marta Benet *Biomarkers and Precision Medicine Unit, Instituto de Investigación Sanitaria La Fe, 46026, Valencia, Spain.
Miriam EstorsThoracic Surgery Service, Hospital Universitario de La Ribera, Valencia, Spain.
Antonio CremadesPathological Anatomy Service, Hospital Universitario de La Ribera, Valencia, Spain.
Óscar Juan-VidalBiomarkers and Precision Medicine Unit, Instituto de Investigación Sanitaria La Fe, 46026, Valencia, Spain.
Julián CarreteroPhisiology Department, University of Valencia, Valencia, Spain.
José Marcelo Galbis-CaravajalThoracic Surgery Service, Hospital Universitario de La Ribera, Valencia, Spain.
Agustín LahozBiomarkers and Precision Medicine Unit, Instituto de Investigación Sanitaria La Fe, 46026, Valencia, Spain. agustin.lahoz@uv.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The tumor microenvironment comprises different cell subsets including myeloid-derived suppressor cells, which exert intratumoral immunosuppression and favor cancer progression. Isolating tumor-infiltrating myeloid-derived suppressor cells (tMDSCs) from human tumor samples remains a challenge. Current methods such as magnetic bead sorting (MACS) or flow cytometry sorting (FACS) present some drawbacks in terms of purity and viability. Here, we have setup an innovative workflow that combines RosetteSep technology and MACS for isolation of tMDSCs from lung cancer biopsies. To evaluate our Rosette-MACS approach, we compared its performance with MACS and FACS. The isolated cells were characterized by flow cytometry, gene expression analysis and proliferation assays for comparison purposes. The results showed that the Rosette-MACS protocol had the highest yield and purity of tMDSCs (79.64% vs 13.30% with FACS and 0.39% with MACS). Furthermore, the functionality of the isolated tMDSCs was tested not only by upregulation of immunosuppressive genes (e.g. ARG1, IDO1, or PD-L1), but also by their capacity to inhibit CD8

Indexed as

Cell SeparationLung NeoplasmsLymphocytes, Tumor-InfiltratingMyeloid-Derived Suppressor CellsArginaseB7-H1 AntigenCD8-Positive T-LymphocytesCell ProliferationFlow CytometryHumansImmunomagnetic SeparationIndoleamine-Pyrrole 2,3,-DioxygenaseTumor MicroenvironmentARG1 protein, humanArginaseB7-H1 AntigenCD274 protein, humanIDO1 protein, humanIndoleamine-Pyrrole 2,3,-DioxygenaseHumanImmunosuppressionIsolationLung cancerMyeloid-derived suppressor cellsTumor-infiltrating

Identifiers

PMID40307421
PMCPMC12044027

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.