Evidence map›Paper›PMID 40305211›Full record

ArticleBiomolecules2025

Oxygen Concentration Plays a Critical Role in Fibrinogen-Mediated Platelet Activation via Inactivation of α

Sophie V L Leonard, Zoe Booth, Leigh Naylor-Adamson, Lewis Bibby, Katie S Wraith, Giordano Pula, Monica Arman, Simon D J Calaminus

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sophie V L LeonardBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.ORCID 0009-0001-4288-5949
Zoe BoothBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.
Leigh Naylor-AdamsonBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.ORCID 0000-0001-5936-9281
Lewis BibbyBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.
Katie S WraithBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.ORCID 0009-0000-7515-8492
Giordano PulaBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.
Monica ArmanBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.
Simon D J CalaminusBiomedical Institute for Multimorbidity, Hull York Medical School, University of Hull, Hull HU6 7RX, UK.

Funding

British Heart Foundation FS/PhD/22/29265Medical Research Council MR/T002573/1
6 · The paper itself

Abstract

In the vascular system, pathological conditions that cause hypoxia are associated with increased platelet activity and thrombosis. Using a platelet spreading assay, we show that severe hypoxia (i.e., 1%), venous (i.e., 5%), and, surprisingly, arterial (i.e., 12%) oxygen concentrations cause a significant reduction in platelet surface area coverage on fibrinogen in comparison to atmospheric oxygen condition (i.e., 21% oxygen), whilst adhesion and spreading on collagen and CRP were not affected. Importantly, the addition of thrombin or zinc restored full platelet spreading on fibrinogen, indicating that the inhibition of platelet spreading on fibrinogen was due to defective integrin activation. Analysis of integrin activation with FACs via PAC-1 staining supported a significant reduction in integrin activation in hypoxic conditions. Interestingly, a fibrinogen matrix prepared at 1%, 5%, or 12% oxygen failed to induce full platelet spreading, even when the experiments were performed at atmospheric oxygen concentration, indicating that the structure and activity of the fibrinogen coating is affected by oxygen. The effect of oxygen on different matrix proteins is critical to understand, as these data clearly demonstrate that collagen and CRP can support platelet activation at all O

Indexed as

Blood PlateletsFibrinogenOxygenPlatelet ActivationPlatelet Glycoprotein GPIIb-IIIa ComplexCollagenHumansPlatelet AdhesivenessCollagenFibrinogenOxygenPlatelet Glycoprotein GPIIb-IIIa Complexactin cytoskeletonfibrinogenhypoxiaintegrin functionoxygenplatelet

Identifiers

PMID40305211
PMCPMC12024578

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.