Evidence map›Paper›PMID 40304906›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Metal Organic Layers-Based Sample Preparation Method for Membrane Proteome Analysis.

Jiang Qianqian, Jiang Bo, Zhang Yukui, Zhang Lihua

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jiang QianqianState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Jiang BoState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China. jiangbo@dicp.ac.cn.ORCID https://orcid.org/0000-0001-5647-6890
Zhang YukuiState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Zhang LihuaState Key Laboratory of Medical Proteomics, National Chromatographic R. & A. Center, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As a central platform for exchange of signaling communication and substance between cells and within cells, mapping of membrane proteome can provide new insights into the pathophysiology of cancer and other diseases, explore potential drug targets and biomarkers for prognosis or diagnosis, and ultimately contribute to the development of precision medicine. However, mass spectrometry-based identification of membrane proteins poses many challenges due to the intrinsic properties of membrane proteins, especially high hydrophobicity and low abundance. Herein, we described a metal organic layers (MOL)-based sample preparation method for membrane proteome analysis in living cells, involving the cell surface engineering process through the exquisite interaction between MOL and phospholipid bilayers on the membrane and the following biomembrane fusion process, providing a simple and robust tool for dissection of membrane proteome with high specificity. This chapter details the preparation of such metal organic layers and enrichment workflow of HeLa membrane proteins.

Indexed as

Membrane ProteinsProteomeProteomicsCell MembraneHeLa CellsHumansLipid BilayersPhospholipidsLipid BilayersMembrane ProteinsPhospholipidsProteomebased identificationEnrichmentMass spectrometryMembrane proteomeMetal organic layers

Identifiers

PMID40304906

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.