ArticleMethods in molecular biology (Clifton, N.J.)2025
Isolation of the Surfaceome from Primary Human Epidermal Melanocytes Using Glycocapture and Shotgun Proteomics.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Exploring the Link Between PACAP Signalling and Hyaluronic Acid Production in Melanoma Progression.International journal of molecular sciences · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melanocytes play a crucial role in skin pigmentation and protection against UV radiation. Understanding the molecular mechanisms regulating melanocyte function is essential for elucidating skin pigmentation disorders and melanoma progression. This protocol details a comprehensive methodology for characterizing the cell surface subproteome of melanocytes and melanoma cell lines, utilizing aminooxy biotinylation followed by mass spectrometry analysis. The initial step involves selectively labeling cell surface proteins with aminooxy biotin on live melanocytes and melanoma cell lines to specifically tag glycoproteins. Labeled proteins are then captured on NeutrAvidin beads. Following multiple wash steps to minimize contamination with non-cell surface proteins, mass spectrometry is employed for quantitative and qualitative analysis of the enriched subproteome, facilitating the identification and characterization of surface proteins. This is a sensitive method for profiling the cell surface subproteome in melanocytes and melanoma cell lines in vitro, offering deeper insights into their molecular composition. This protocol holds promise for advancing our understanding of melanoma and may aid in identifying potential therapeutic targets for melanoma treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.