Evidence map›Paper›PMID 40304894›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2025

Evaluation of the APTIMA Combo 2™ kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semen.

Thibaut Lutz, Robin Chautard, Maxime Lafontaine, Merve Genc, Arabella Touati, Sabine Pereyre, Olivia Peuchant, Cécile Bébéar, Fabien Garnier, Sébastien Hantz

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Laboratory detection methods for Chlamydia trachomatis infection in children: a review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Review
  2. Rapid and simple detection of Chlamydia trachomatis and Neisseria gonorrhoeae using the EasyNAT CT/NG assay based on cross-priming amplification.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Thibaut LutzReproductive Biology Department, CHU Limoges, Limoges, France.
Robin ChautardReproductive Biology Department, CHU Limoges, Limoges, France.
Maxime LafontaineReproductive Biology Department, CHU Limoges, Limoges, France.
Merve GencReproductive Biology Department, CHU Limoges, Limoges, France.
Arabella TouatiBacteriology Department, National Reference Center for Bacterial STIs, CHU Bordeaux, Bordeaux, France.
Sabine PereyreBacteriology Department, National Reference Center for Bacterial STIs, CHU Bordeaux, Bordeaux, France.
Olivia PeuchantBacteriology Department, National Reference Center for Bacterial STIs, CHU Bordeaux, Bordeaux, France.
Cécile BébéarBacteriology Department, National Reference Center for Bacterial STIs, CHU Bordeaux, Bordeaux, France.
Fabien GarnierBacteriology-Virology-Hygiene Department, CHU Limoges, Limoges, France.
Sébastien HantzBacteriology-Virology-Hygiene Department, CHU Limoges, Limoges, France. sebastien.hantz@chu-limoges.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2™ kit for screening cryopreserved sperm samples before donation.

Indexed as

Chlamydia InfectionsChlamydia trachomatisGonorrheaNeisseria gonorrhoeaeReagent Kits, DiagnosticSemenCryopreservationHumansLimit of DetectionMaleSemen PreservationSensitivity and SpecificityReagent Kits, DiagnosticChlamydia trachomatisCryopreserved semenNAATNeisseria gonorrhoeae

Identifiers

PMID40304894
PMCPMC12321919

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.