ArticleJournal of fluorescence2025
A Simple Fluorescent Probe for Biothiols Detection and Imaging of Living Cells In Vivo Based on a Hemicyanine Derivative.
Article in Journal of fluorescence, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- A Novel Pyrazoline-Based Fluorescent Turn-On Probe for Biothiol Detection and Live-Cell Imaging.Journal of fluorescence · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Biothiols play essential roles in various biological processes and are closely associated with diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. To monitor biothiols in organisms and living cells, we developed a novel intramolecular charge transfer (ICT)-based probe, hemicyanine-2,4-dinitrobenzenesulfonamide(HCD), by coupling a hemicyanine dye with 2,4-dinitrobenzenesulfonyl chloride.The HCD probe exhibits remarkable sensitivity, with low detection limits of 0.32 µM for Cys, 0.72 µM for Hcy, and 0.27 µM for GSH, and demonstrates high selectivity for biothiols in the presence of various interfering species. The detection mechanism was thoroughly validated using high-resolution mass spectrometry (HRMS), UV-Vis spectroscopy, fluorescence spectroscopy and Density Functional Theory (DFT) calculation. Furthermore, the applicability of HCD was successfully demonstrated in human urine samples and in vivo settings using RAW264.7 cells, confirming its potential as a powerful tool for monitoring endogenous biothiols in biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.