ArticleCombinatorial chemistry & high throughput screening2026
High-Content Screening of Wind-Dampness Dispelling Traditional Chinese Medicines against Podocyte EMT Induced by IgA1.
Article in Combinatorial chemistry & high throughput screening, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveImmunoglobulin A (IgA) Nephropathy (IgAN) is characterized by pIgA1 dep-osition in the glomerular mesangium, resulting in podocyte Epithelial-to-Mesenchymal Transi-tion (EMT). Traditional Chinese Medicines (TCMs) have been used for the treatment of IgAN for several years and have demonstrated positive efficacy. The present study aimed to establish a High-Content Screening (HCS) method for identifying wind-dampness dispelling TCM extracts that can mitigate podocyte EMT induced by pIgA1. MATERIAL AND
methodsIgA1 from IgAN patients was used to establish a podocyte EMT model. The expression of EMT markers, including desmin, podocalyxin, and podocin, was assessed by Immunocytofluorescence (IF). An image-based HCS method was established to identify winddampness dispelling TCMs with the anti-EMT activity of podocyte EMT by automatic acquisition and processing of dual-fluorescent labeled images.
resultsA total of 21 wind-dampness-dispelling TCM extracts were screened using the HCS system, leading to the identification of eight wind-dampness-dispelling TCM extracts exhibiting anti-EMT activity. These eight extracts inhibited the pIgA-induced expression of desmin while upregulating the expression of podocalyxin and podocin.
conclusionBy quantifying the changes in the expression of EMT markers during pIgA1- induced EMT, this study successfully identified wind-dampness dispelling TCM extracts with anti- EMT properties using an HCS system. In addition, the proposed approach presents a novel avenue for the identification of wind-dampness-dispelling TCMs for the treatment of pIgA1- induced EMT.
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