Evidence map›Paper›PMID 40303009›Full record

ArticleTransboundary and emerging diseases2024

Establishment of a Triplex qPCR Assay for Differentiating Highly Virulent Genotype I Recombinant Virus From Low-Virulence Genotype I and Genotype II African Swine Fever Viruses Circulating in China.

Leilei Ding, Tao Ren, Guoxia Bing, Zhigang Wang, Baoyue Wang, Jianqiang Ni, Yuliang Liu, Rui Zhao, Yuanmao Zhu, Fang Li and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Leilei DingState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Tao RenState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Guoxia BingChina Animal Disease Control Center, Beijing 100125, China.
Zhigang WangState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Baoyue WangChina Animal Disease Control Center, Beijing 100125, China.
Jianqiang NiChina Animal Disease Control Center, Beijing 100125, China.
Yuliang LiuChina Animal Disease Control Center, Beijing 100125, China.
Rui ZhaoState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Yuanmao ZhuState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Fang LiState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Renqiang LiuState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Qiang FuCollege of Veterinary Medicine Xinjiang Agricultural University, Urumqi 830052, China.
Zhijun TianState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Zhigao BuState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.
Encheng SunState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID https://orcid.org/0000-0002-4750-2329
Dongming ZhaoState Key Laboratory for Animal Disease Control and Prevention National High Containment Facilities for Animal Disease Control and Prevention National African Swine Fever Para-reference Laboratory Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID https://orcid.org/0000-0001-7161-6214

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African swine fever virus (ASFV) poses serious threats to the global swine industry, food safety, and the economy. Since August 2018, different types of ASFVs have successively emerged in China, making ASF diagnostics more challenging. The highly virulent genotype I recombinant virus has gradually become the prevalent dominant strain and is identified by sequencing several of its genes, which is time-consuming and expensive. Here, we developed a triplex real-time quantitative PCR (qPCR) assay based on the ASFV B646L, X64R, and MGF_360-14L genes to differentiate highly virulent genotype I recombinant viruses from low-virulence genotype I and genotype II viruses in China. This method has high sensitivity and a limit of detection of 10 copies/reaction for standard plasmids, as well as good specificity without cross-reactions with the viral nucleic acids of porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), pseudorabies virus (PRV), porcine circovirus 2 (PCV 2), porcine circovirus 3 (PCV 3), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), or porcine rotavirus (PoRV). Importantly, triplex qPCR can be used to quickly and accurately evaluate clinical samples and cell cultures infected with highly virulent genotype I virus, low-virulence genotype I virus, or genotype II virus. Thus, triplex qPCR provides an alternative tool for ASF surveillance in China.

Indexed as

African Swine FeverAfrican Swine Fever VirusMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionAnimalsChinaGenotypeSensitivity and SpecificitySwineVirulenceAfrican swine fever virusdifferential detectiongenotype IIhighly virulent genotype Ilow virulent genotype Itriplex real-time quantitative PCR

Identifiers

PMID40303009
PMCPMC12017118

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.