ArticleChem & bio engineering2025
Crystallization-Assisted Asymmetric Synthesis of Enantiopure Amines Using Membrane-Immobilized Transaminase.
Article in Chem & bio engineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Engineering of ω-transaminases for advanced applications.Applied microbiology and biotechnology · 2026Review
- Crystallization-Assisted Asymmetric Synthesis of Enantiopure Amines Using Membrane-Immobilized Transaminase.Chem & bio engineering · 2025Article
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Authors and funding
9 authors.
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Abstract
The production of active pharmaceutical ingredients (APIs) requires enantiopure chiral amines, for which greener synthesis processes are needed. Transaminases (TAs) are enzymes that catalyze the enantioselective production of chiral amines from prochiral ketones through transamination under mild conditions. Yet, industrial applications of biocatalytic transamination remain currently hindered by the limited stability of soluble enzymes and by the unfavorable thermodynamic equilibrium of targeted asymmetric reactions. Enzyme immobilization can be applied to address stability, recoverability, and reusability issues. In the perspective of process intensification, we chose to immobilize TAs on polymeric (polypropylene) membranes. In the asymmetric synthesis of (R)-2-fluoro-α-methylbenzylamine ((R)-FMBA), such membrane-immobilized TAs exhibited superior specific activity and stability compared with soluble TAs; they also outperformed TAs immobilized on resins. The reaction yield remained, however, limited by thermodynamics. To further enhance the synthesis yield, the reaction was coupled with the
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Registered trials
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