Evidence map›Paper›PMID 40302746›Full record

ArticleTransboundary and emerging diseases2025

Real-Time Reverse Transcription Multienzyme Isothermal Rapid Amplification for Rapid Detection of African Horse Sickness Virus.

Chaohua Huang, Jianchang Wang, Zhouxi Ruan, Jiang Wu, Yanxing Lin, Chenfu Cao, Junxing Yang, Qiaoyu Weng, Ye Jin, Peng Chen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transboundary and emerging diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chaohua HuangLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.ORCID https://orcid.org/0009-0006-2863-3354
Jianchang WangLaboratory of Animal Quarantine, Technology Center of Shijiazhuang Customs, Shijiazhuang 050051, Hebei Province, China.
Zhouxi RuanLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.
Jiang WuLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.
Yanxing LinLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.
Chenfu CaoLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.
Junxing YangLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.
Qiaoyu WengDepartment of Product Development, Shenzhen Biolove Technology Co., Ltd, Shenzhen 518120, China.
Ye JinDepartment of Product Development, Hu Nan Project Bioscience Ltd, Changsha 410013, China.
Peng ChenDepartment of Product Development, Shenzhen Biolove Technology Co., Ltd, Shenzhen 518120, China.
Qunyi HuaLaboratory of Animal Quarantine, Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Customs, Shenzhen 518045, Guangdong Province, China.ORCID https://orcid.org/0009-0009-5122-6169

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African horse sickness (AHS) is an acute infectious disease of equids caused by the AHS virus (AHSV), which can cause up to 90% mortality in naive horses. Reliable and rapid diagnosis is crucial for the surveillance and control of AHSV. As one of the AHSV detection methods recommended by World Organization for Animal Health (WOAH), the RT-qPCR assay has the drawbacks such as complex operation, expensive instruments, and long detecting time, which limit its application in simple laboratories or outdoors. In this study, a real-time reverse transcription multienzyme isothermal rapid amplification (RT-MIRA) assay was established to detect AHSV. Primers and exo-probes were designed, synthesized, and screened based on the conserved regions of the AHSV

Indexed as

African Horse SicknessAfrican Horse Sickness VirusMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesAnimalsHorsesReal-Time Polymerase Chain ReactionReverse TranscriptionSensitivity and Specificityafrican horse sickness virusrapid detectionreal-time RT-MIRAscreening

Identifiers

PMID40302746
PMCPMC12016855

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.