Evidence map›Paper›PMID 40301686›Full record

ArticleBritish journal of cancer2025

POU6F2 promotes liver metastasis of gastric adenocarcinoma by dual mechanism of transcriptional upregulation of SNAI1 and IGF2/PI3K/AKT signaling-induced conversion of hepatic stellate cells into cancer-associated fibroblasts.

Chunxiao Yang, Zhiqing Gao, Ruiming Tang, Lihuan Zhou, Ping Zhou, Wangpan Shi, Dong Ren, Han Chen, Zhuojun Zhang, Xiaoyi Xie and 9 more

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Article in British journal of cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

19 authors.

Chunxiao Yang *The Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Zhiqing Gao *Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Disease, Guangzhou Medical University, Guangzhou, China.
Ruiming Tang *The Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Lihuan Zhou *The Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Ping ZhouGuangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Disease, Guangzhou Medical University, Guangzhou, China.
Wangpan ShiDepartment of Pathology, University of California San Diego Health System, San Diego, CA, USA.
Dong RenDepartments of Pathology, University of California Irvine Medical Center, Orange, CA, USA.
Han ChenThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Zhuojun ZhangThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Xiaoyi XieThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Jiaqian LinThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Yingming YeDepartment of Pathogen Biology and Immunology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, China.
Zhengfu FengThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Xiaoli FengThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China.
Yaoming HeDepartment of Gastrointestinal Surgery, Jiangmen Central Hospital, Jiangmen, China.
Yaofeng ZhiClinical Experimental Center, Jiangmen Key Laboratory of Precision and Clinical Translation Medicine, Jiangmen Clinical Medical College of Guangdong Medical University, Jiangmen Central Hospital, Jiangmen, China.
Dongmei LiuClinical Experimental Center, Jiangmen Key Laboratory of Precision and Clinical Translation Medicine, Jiangmen Clinical Medical College of Guangdong Medical University, Jiangmen Central Hospital, Jiangmen, China.
Xin ZhangClinical Experimental Center, Jiangmen Key Laboratory of Precision and Clinical Translation Medicine, Jiangmen Clinical Medical College of Guangdong Medical University, Jiangmen Central Hospital, Jiangmen, China. zhangx45@mail3.sysu.edu.cn.
Lili JiangThe Affiliated Qingyuan Hospital (Qingyuan People's Hospital), Guangzhou Medical University, Qingyuan, China. jianglili@gzhmu.edu.cn.ORCID http://orcid.org/0000-0003-2361-5975

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundActivation of cancer-associated fibroblasts (CAFs) plays an important role in tumor metastasis. The purpose of this study is to investigate the role of POU6F2 in conversion of hepatic stellate cells (HSCs) into CAFs in liver metastasis of gastric adenocarcinoma (GAC).

methodsPOU6F2 expression was examined by real-time PCR, Western blot and immunohistochemical staining. The functional roles of POU6F2 in GAC liver metastasis were investigated both cellular experiments in vitro and in vivo using a mouse model of subcutaneous splenic injection. ChIP and ELISA assays were used to explore the underlying molecular mechanism of POU6F2 in liver metastasis of GAC.

resultsHere we reported that POU6F2 was upregulated in GAC tissue with liver metastasis, which predicted poor early liver metastasis. Upregulating POU6F2 promoted EMT, invasion and migration of GAC cells in vitro, and the liver metastasis of GAC cells in vivo. Mechanic investigation further revealed that upregulating POU6F2 promoted the invasion and metastasis of GAC by transcriptional upregulation of EMT-inducer SNAI1, and promoting the conversion of HSCs into CAFs dependent on transcriptional upregulation of IGF2-induced activation of PI3K/AKT signaling.

conclusionOur findings uncover a novel dual mechanism by which POU6F2 promotes liver metastasis of GAC.

Indexed as

AdenocarcinomaCancer-Associated FibroblastsHepatic Stellate CellsInsulin-Like Growth Factor IILiver NeoplasmsSnail Family Transcription FactorsStomach NeoplasmsAnimalsCell Line, TumorCell MovementEpithelial-Mesenchymal TransitionFemaleGene Expression Regulation, NeoplasticHumansMaleMiceIGF2 protein, humanInsulin-Like Growth Factor IIPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSNAI1 protein, humanSnail Family Transcription Factors

Identifiers

PMID40301686
PMCPMC12238622

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.