Evidence map›Paper›PMID 40301238›Full record

ArticleAMB Express2025

Method for automated high performance closed batch cultivation of gas-utilizing methanogens.

Walter Hofmann, Marco Orthofer, Nicolás Salas Wallach, Aquilla Ruddyard, Markus Ungerank, Christian Paulik, Simon K-M R Rittmann

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Valine overproduction inMetabolic engineering communications · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Walter Hofmann *Archaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Djerassiplatz 1, 1030, Wien, Austria.
Marco Orthofer *Institute for Chemical Technology of Organic Materials, Johannes Kepler Universität Linz, Linz, Austria.
Nicolás Salas WallachArchaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Djerassiplatz 1, 1030, Wien, Austria.
Aquilla RuddyardArchaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Djerassiplatz 1, 1030, Wien, Austria.
Markus UngerankCreonia e.U, Perg, Austria.
Christian PaulikInstitute for Chemical Technology of Organic Materials, Johannes Kepler Universität Linz, Linz, Austria.
Simon K-M R RittmannArchaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Djerassiplatz 1, 1030, Wien, Austria. simon.rittmann@univie.ac.at.ORCID http://orcid.org/0000-0002-9746-3284

Funding

Österreichische Forschungsförderungsgesellschaft 888509
6 · The paper itself

Abstract

To advance the utilization of microbial cell factories in gas fermentation processes, their physiological and biotechnological characteristics must be understood. Here, we report on the construction and operation of a novel device, the Gas and Pressure Controller (GPC), which is specifically designed for the automated control of the headspace gas pressure of closed cultivation bottles and facilitates automated gassing, sparging, monitoring and regulation of the headspace volume operated in closed batch cultivation mode in real time.As proof of concept, the physiological and biotechnological characteristics of four autotrophic, hydrogenotrophic methanogenic archaea were examined to quantify novel physiological limits through the elimination of gas limitation during growth and methane formation. We determined unprecedented high maximum specific methane productivity (qCH

Indexed as

AnaerobeBiotechnologyGas fermentationMethanogenic archaeaMicrobiologyMicroorganisms

Identifiers

PMID40301238
PMCPMC12040808

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.