Evidence map›Paper›PMID 40300606›Full record

ArticleCell reports methods2025

Fluorescence microscopy through scattering media with robust matrix factorization.

Zijun Gao, Zhi Ling, Wenhao Liu, Keyi Han, Hongmanlin Zhang, Xuanwen Hua, Edward A Botchwey, Shu Jia

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zijun GaoLaboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Zhi LingLaboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Wenhao LiuLaboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Keyi HanLaboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Hongmanlin ZhangWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Xuanwen HuaLaboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Edward A BotchweyWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Shu JiaLaboratory for Systems Biophotonics, Georgia Institute of Technology, Atlanta, GA 30332, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA; Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: shu.jia@gatech.edu.

Funding

Toward Systems Biophotonics: Imaging Biology across High Dimensions and ScalesR35GM124846 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI Shu Jia · 2017 to 2026
$4.0M
NIGMS NIH HHS R35 GM124846
6 · The paper itself

Abstract

Biological tissues, as natural scattering media, inherently disrupt structural information, presenting significant challenges for optical imaging. Complex light propagation through tissue severely degrades image quality, limiting conventional fluorescence imaging techniques to superficial depths. Extracting meaningful information from random speckle patterns is, therefore, critical for deeper tissue imaging. In this study, we present RNP (robust non-negative principal matrix factorization), an approach that enables fluorescence microscopy under diverse scattering conditions. By integrating robust feature extraction with non-negativity constraints, RNP effectively addresses challenges posed by non-sparse signals and background interference in scattering tissue environments. The framework operates on a standard epi-fluorescence platform, eliminating the need for complex instrumentation or precise alignment. The results from imaging scattered cells and tissues demonstrate substantial improvements in robustness, field of view, depth of field, and image clarity. We anticipate that RNP will become a valuable tool for overcoming scattering challenges in fluorescence microscopy and driving advancements in biomedical research.

Indexed as

Image Processing, Computer-AssistedAlgorithmsAnimalsHumansMiceMicroscopy, FluorescenceOptical ImagingScattering, RadiationCP: ImagingCP: Systems biologyfluorescence microscopynon-invasive imagingscattering imagingtissue imaging

Identifiers

PMID40300606
PMCPMC12146666

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.