Evidence map›Paper›PMID 40300591›Full record

ArticleThe American journal of tropical medicine and hygiene2025

Characterization of Transstadial Transmission of Rickettsia Amblyommatis in Haemaphysalis Longicornis Using Optimized Artificial Membrane Feeding System.

Justin Le, Molly B Thompson, Wan-Yi Yen, Smruti Mishra, David Carlson, Andrew S Handel, Erich R Mackow, Jorge L Benach, Ilia Rochlin, Hwan Keun Kim

Abstract read
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Justin LeCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
Molly B ThompsonCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
Wan-Yi YenCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
Smruti MishraCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
David CarlsonInstitute for Advanced Computational Science, Stony Brook University, Stony Brook, New York.
Andrew S HandelDepartment of Pediatrics, Stony Brook University, Stony Brook, New York.
Erich R MackowCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
Jorge L BenachCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
Ilia RochlinCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.
Hwan Keun KimCenter for Infectious Diseases, Stony Brook University, Stony Brook, New York.

Funding

Molecular basis of immunity to tick-borne rickettsiosesR01AI152208 · NIAID · STATE UNIVERSITY NEW YORK STONY BROOK · PI KIM, HWAN KEUN · 2021 to 2025
$2.8M
Host-pathogen-vector interactions of R. amblyommatis for spotted fever pathogenesisR21AI169287 · NIAID · STATE UNIVERSITY NEW YORK STONY BROOK · PI KIM, HWAN KEUN · 2022 to 2023
$425k
NIAID NIH HHS R01 AI152208NIAID NIH HHS R21 AI169287
6 · The paper itself

Abstract

Ticks are blood-feeding arthropods and serve as vectors and reservoirs for diverse pathogens. Recent environmental changes have triggered the invasion of ticks into new geographical areas, prompting a public health alert for increased risk of tick-borne diseases. Amblyomma (A.) americanum (lone star tick) has emerged as the most common human-biting tick species in the eastern United States. Amblyomma americanum transmits multiple pathogens, including Rickettsia (R.) amblyommatis, the suspected cause of mild spotted fever rickettsiosis. As an invasive tick species, Haemaphysalis (H.) longicornis (longhorned tick) has rapidly invaded and expanded to high densities in the eastern United States. Haemaphysalis longicornis and A. americanum often share the habitat with preferential feeding on mid- to large-sized animals, such as white-tailed deer. This sympatric association of H. longicornis with A. americanum raises the potential for H. longicornis to acquire pathogens native to A. americanum during blood-feeding on the same host. In its native ranges, H. longicornis transmits R. japonica and R. heilongjiangensis. However, it remains unclear whether H. longicornis can transmit R. amblyommatis abundantly present in A. americanum in the United States. Using artificial membrane feeding, we establish that R. amblyommatis can stably colonize H. longicornis without altering feeding and molting behaviors. Transovarial transmission of R. amblyommatis did not occur in parthenogenetic H. longicornis. However, R. amblyommatis successfully invaded the midgut and salivary glands of H. longicornis, key organ tissues of rickettsial replication and horizontal transmission. Our results suggest that H. longicornis may serve as a vector, but not as a reservoir, for R. amblyommatis transmission.

Indexed as

AmblyommaArachnid VectorsIxodidaeRickettsiaRickettsia InfectionsAnimalsDeerFeeding BehaviorFemaleHaemaphysalis longicornis

Identifiers

PMID40300591
PMCPMC12225575

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.