Evidence map›Paper›PMID 40299219›Full record

ArticleMarine biotechnology (New York, N.Y.)2025

Sterilization of Channel Catfish (Ictalurus punctatus) via Overexpression of bax Gene Regulated by a Tet-off System in the Primordial Germ Cells.

Zhi Ye, Ahmed Elaswad, Guyu Qin, Dongdong Zhang, Baofeng Su, Karim Khalil, Zhenkui Qin, Nermeen Y Abass, Qi Cheng, Ramjie Odin and 9 more

Abstract read
PubMed Publisher
In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Zhi Ye *Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, Ocean University of China, Sanya, 572024, China. yezhi@ouc.edu.cn.
Ahmed Elaswad *School of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Guyu QinSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Dongdong ZhangSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Baofeng SuSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Karim KhalilSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Zhenkui QinSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Nermeen Y AbassSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Qi ChengSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Ramjie OdinSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Khoi VoSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Nathan BackenstoseSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
David DrescherSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Mei ShangSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Hanbo LiSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Dan ZhangSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
William S BuggSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Kamal GoshSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Rex A DunhamSchool of Fisheries, Aquaculture, and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.

Funding

US Department of Agriculture Biotechnology Risk Assessment Grant Program 2009-33522-05774
6 · The paper itself

Abstract

Transgenic technologies have been used for genetic improvement of catfish performance with notable success. However, these developments are useless from a commercialization standpoint without extremely efficient confinement. Transgenic sterilization has the potential to accomplish 100% reproductive confinement and avoid genetic exchange between transgenic or domestic genotypes and wild populations. The present study reports a novel sterilization method for channel catfish by overexpressing the pro-apoptosis gene bax, specifically in the primordial germ cells, to inhibit their proliferation. Three transgenic constructs were electroporated into channel catfish one-cell embryos, including Nanos-nanos, Nanos-dnd, and Dazl-vasa. Transgene integration, gonad development, and sex ratio were evaluated in P

Indexed as

bcl-2-Associated X ProteinFish ProteinsGerm CellsIctaluridaeSterilization, ReproductiveAnimalsAnimals, Genetically ModifiedElectroporationFemaleGonadsMaleSex RatioTransgenesbcl-2-Associated X ProteinFish ProteinsApoptosisBaxChannel catfishPrimordial germ cellTransgenic sterilization

Identifiers

PMID40299219

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.