ArticleAntibiotics (Basel, Switzerland)2025
Design and Validation of a Simplified Method to Determine Minimum Bactericidal Concentration in Nontuberculous Mycobacteria.
Article in Antibiotics (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Carbon Quantum Dots-Enhanced Polyvinyl Alcohol: A Comprehensive Approach to Food Preservation.Comprehensive reviews in food science and food safety · 2026Review
- Employment of a Resazurin Viability-Based Assay for Minimum Inhibitory and Bactericidal Concentration Determination.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Synthesis, Topological, and Biological Studies of a Novel One-Dimensional Hg(II) Coordination Polymer With Antibacterial and Anticancer Potentials.Bioinorganic chemistry and applications · 2026Article
- Evaluation of Antimicrobial and Antibiofilm Activity ofMicroorganisms · 2025Article
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Authors and funding
11 authors.
Funding
Abstract
BACKGROUND/
objectivesNontuberculous mycobacteria (NTM) infections are rising, particularly those by
methodsFirst, we assessed the stability of the antibiotics included in SLOWMYCOI Sensititre™ microtiter plates. Five strains of MAC were studied comparing the minimum inhibitory concentrations (MICs) of those preincubated for seven days vs. non-incubated plates. Then, we evaluated the percentage of reproducibility of MBC values using two methods, reincubation and subculturing (standard or traditional method) in 30 MAC isolates. Finally, we validated the reincubation method and prospectively determined the MBC values of the 229 NTM clinical strains.
resultsAntibiotic stability: The MIC was equivalent after 7 and 14 days for all the antibiotics, except rifampicin, for which the MIC increased by 2- to 3-fold after preincubation. Reincubation method: The percentage of reproducibility of the MBC values between the two methods was 95.2% (range 76.6% to 100%). Prospective validation: MBC/MIC ratios revealed differential bactericidal activity for most antibiotics according to the different species, being bactericidal in
conclusionsPreincubation of Sensititre™ microtiter plates did not alter the MIC values of the antibiotics included except for rifampicin, suggesting a loss of activity. MBC determination can be easily performed by the Reincubation method presented. MBC values provide useful additional information regarding MIC values since the MBC/MIC ratio reveals whether antibiotics have bactericidal or bacteriostatic activity according to the species, which is pivotal for selecting the most adequate antibiotic combination to ensure efficient treatment management.
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