ArticleAntibiotics (Basel, Switzerland)2025
Development and Validation of a Multiplex Real-Time PCR Assay for Rapid Screening of Main Carbapenemase Genes in Clinical Isolates and Surveillance Samples.
Article in Antibiotics (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Validation of Hexaplex Recombinase Polymerase Amplification Assay for the Detection of Carbapenemase Genes in Klebsiella pneumoniae Isolates From Pediatric Inpatients.Journal of clinical laboratory analysis · 2026Article
- Article
- Gram-negative bloodstream infections in pediatric patients: antimicrobial resistance and factors associated with mortality.Frontiers in pediatrics · 2026Article
- Molecular Characterization of Cocarriage Carbapenemase Genes in ClinicalInternational journal of microbiology · 2026Article
- Antibiotic susceptibility patterns of clinical isolates of salmonella species producing extended spectrum beta lactamases as predictor of multidrug resistance in a tertiary hospital, Southeastern Nigeria.BMC infectious diseases · 2025Article
- Rapid 65-min SYBR-Green PCR Assay for Carbapenem ResistantMicroorganisms · 2025Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
objectivesCarbapenem-resistant
methodsPrimers and probes targeted at genes encoding carbapenemases
resultsA good linear correlation between the fluorescence values in the real-time PCR and the log
conclusionsThe assay offers a rapid, sensitive and specific method for the screening of major carbapenemase genes, providing an effective tool for surveillance and infection control in clinical settings. The DNA extraction-free protocol converts this method into a good alternative for screening in 24/7 clinical laboratories. Further multiplexing to target other resistance genes, on demand, could add potential benefits to this laboratory-developed method.
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