Distinct blood and oral microbiome profiles reveal altered microbial composition and functional pathways in myocardial infarction patients.

Introduction: The blood microbiome, increasingly recognized as a distinct microbial niche, may originate partly from oral translocation. We systematically compared circulating and oral microbiome profiles between healthy individuals and myocardial infarction (MI) patients to identify disease-associated signatures. Methods: The current study recruited 20 participants, including 10 healthy controls and 10 patients with MI. Blood and Saliva samples were collected from each participant to analyze the association between blood and oral microbiome in MI patients using 16S rRNA gene sequencing. Results: The blood microbiome showed significantly greater alpha diversity than the oral microbiome (p<0.05), but beta diversity did not differ significantly. The blood microbiome in MI patients had higher levels of Firmicutes, Bacteroidota, Actinobacteriota, genus Bacteroides, and lower Proteobacteria, whereas the oral microbiome was dominated by Firmicutes, Bacteroidota, Veillonella, and Prevotella_7. LEfSe analysis revealed distinct blood microbial taxa-Actinobacteria in MI patients and Enterobacterales in controls. In contrast, the oral microbiota of healthy subjects was enriched in Rothia, Micrococcaceae, and Micrococcales, while no distinct taxa were associated with MI. Both blood and oral microbiomes showed significant functional pathway differences (KEGG) between groups. Additionally, microbiome signatures significantly correlated with clinical and demographic markers. Discussion: Our study demonstrates that the blood harbors a distinct microbiome characterized by specific microbial taxa and functional pathways rather than merely reflecting oral bacterial translocation. These findings suggest that the circulating microbiome may play an active role in the pathology of MI. Furthermore, we identified significant associations between these microbial signatures and clinical disease markers. This highlights the potential importance of the blood microbiome in understanding the mechanisms underlying MI and its diagnostic or therapeutic implications.