Evidence map›Paper›PMID 40296106›Full record

ArticleBMC veterinary research2025

Development of a triplex TaqMan probe-based real-time PCR assay for simultaneous detection of swine influenza virus, porcine reproductive and respiratory syndrome virus, and porcine circovirus type 2.

MengJie Wang, Yu Pan, Wengjie Ma, Xingrong Wang, Changyou Xia, Yue Wang

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

MengJie WangState Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Yu PanState Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Wengjie MaState Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Xingrong WangState Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Changyou XiaState Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. xiachangyou@caas.cn.
Yue WangState Key Laboratory for Animal Disease Control and Prevention, National Poultry Laboratory Animal Resource Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. vetyuewang@163.com.

Funding

Central Government-guided Local Science and Technology Development Project ZY04JD03Central Public-interest Scientific Institution Basal Research Fund 1610302022018National Center of Technology Innovation for Pigs NCTIP-XD/C09
6 · The paper itself

Abstract

backgroundPorcine respiratory disease complex poses a substantial economic challenge for the swine farms due to its prevalence in pig farming environments. The disease is primarily caused by viral pathogens such as swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus type 2 (PCV2). Given the high incidence and morbidity associated with these viruses, effective control strategies rely on rapid and accurate diagnosis.

resultsTo this end, we developed and validated a triplex TaqMan probe-based real-time PCR assay for the simultaneous detection and differentiation of SIV, PRRSV, and PCV2. This assay demonstrated high specificity, with no observed cross-reactivity between the target viruses. The assay's sensitivity was determined to be 100 copies/µL for SIV and 10 copies/µL for PRRSV and PCV2. Comparison with a national standard detection method using 110 clinical samples revealed 100% agreement, confirming the reliability of the newly developed assay for clinical diagnostic applications.

conclusionsGiven the zoonotic potential of SIV, this assay not only provides a valuable diagnostic tool for veterinary medicine but also contributes to enhanced public health surveillance efforts.

Indexed as

Circoviridae InfectionsCircovirusMultiplex Polymerase Chain ReactionOrthomyxoviridae InfectionsPorcine respiratory and reproductive syndrome virusReal-Time Polymerase Chain ReactionSwine DiseasesAnimalsPorcine Reproductive and Respiratory SyndromeReproducibility of ResultsSensitivity and SpecificitySwineDetectionPCV2PRRSVReal-time PCRSIV

Identifiers

PMID40296106
PMCPMC12038986

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.