Evidence map›Paper›PMID 40295455›Full record

ArticleMikrochimica acta2025

Colorimetric determination of tyrosinase activity and high-throughput screening of inhibitors based on in-situ formation of gold nanoparticles.

Guohua Zhou, Zhicheng Liu, Yuning Huang, Rufei Yang, Xin Guo, Yongmei Jia, Peilian Liu, Zhiguo Li, Wenjuan Cui

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Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Guohua ZhouKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China. ghzhou@lingnan.edu.cn.
Zhicheng LiuKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China.
Yuning HuangKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China.
Rufei YangKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China.
Xin GuoKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China.
Yongmei JiaKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China. jymchemistry@163.com.
Peilian LiuKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China. liupeilian2010@126.com.
Zhiguo LiKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China.
Wenjuan CuiKey Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, , Lingnan Normal University, Zhanjiang, 524048, Guangdong, China.

Funding

Characteristic Innovation Project of Guangdong 2023ZDZX2037Characteristic Innovation Project of University in Guangdong Province 2023KTSCX073
6 · The paper itself

Abstract

A colorimetric method is presented for detecting tyrosinase activity and screening inhibitors, leveraging the in-situ formation of gold nanoparticles mediated by catechol. The active tyrosinase converses catechol into o-quinone leading to changes in both the size of AuNPs and their corresponding UV-Vis absorption spectrum as well as visual color. This enzyme-mediated alteration can be modulated by tyrosinase inhibitors through chemical regulation of enzyme activity. Based on these, facile detection of tyrosinase and high-throughput screening for its inhibitors can be achieved simply by visually observing color changes in solution. The proposed method is characterized by its sensitivity, simplicity, rapidity, and accuracy, allowing for quantitative determination of tyrosinase within the range 0.5 to 25 U/mL, with a detection limit of 0.038 U/mL. Furthermore, visual or instrumental observation of the color changes in gold nanoparticles enables high-throughput screening of inhibitors and calculation of their IC50 values. We anticipate that this approach will significantly contribute to tyrosinase research and facilitate the development, discovery, and high-throughput screening of effective inhibitors.

Indexed as

ColorimetryEnzyme InhibitorsGoldHigh-Throughput Screening AssaysMetal NanoparticlesMonophenol MonooxygenaseCatecholsLimit of DetectioncatecholCatecholsEnzyme InhibitorsGoldMonophenol MonooxygenaseColorimetric detectionGold nanoparticleHigh-throughput screeningIn-situ formationTyrosinase

Identifiers

PMID40295455

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