Evidence map›Paper›PMID 40294830›Full record

ArticleMatrix biology : journal of the International Society for Matrix Biology2025

Brain vascular basement membrane: Comparison of human and mouse brain at the transcriptomic and proteomic levels.

Nan Zhao, Alexander F Pessell, Tracy D Chung, Peter C Searson

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Matrix biology : journal of the International Society for Matrix Biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nan ZhaoInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA.
Alexander F PessellDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Tracy D ChungDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Peter C SearsonInstitute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, 21218, USA; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA. Electronic address: searson@jhu.edu.

Funding

Tech Core 2U54CA268083 · NCI · JOHNS HOPKINS UNIVERSITY · PI Pei-Hsun wu · 2022 to 2026
$10.2M
Mechanisms of cerebrovascular barrier dysfunction caused by APP and PSEN1 mutations and amyloid beta exposureR01NS106008 · NINDS · JOHNS HOPKINS UNIVERSITY · PI SEARSON, PETER C · 2019 to 2023
$1.9M
Engineering three-dimensional perfusable microphysiological models of the human inner blood-retina barrierR01EY035853 · NEI · DUKE UNIVERSITY · PI Sharon Gerecht · 2024 to 2026
$1.9M
Reverse engineering zonation-specific and age-specific iPSC-derived cerebrovascular models based on transcriptomic profiling of the human brainR61HL154252 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HEIMAN, MYRIAM, SEARSON, PETER C · 2021 to 2022
$1.6M
Reverse engineering zonation-specific and age-specific iPSC-derived cerebrovascular models based on transcriptomic profiling of the human brainR33HL154252 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI HEIMAN, MYRIAM, SEARSON, PETER C · 2023 to 2025
$1.3M
NCI NIH HHS U54 CA268083NEI NIH HHS R01 EY035853NHLBI NIH HHS R33 HL154252NHLBI NIH HHS R61 HL154252NINDS NIH HHS R01 NS106008
6 · The paper itself

Abstract

The cerebrovascular basement membrane (BM) is a key component of the blood-brain barrier (BBB). The BM provides structural support for brain microvascular endothelial cells and the supporting cells of the neurovascular unit, and facilitates cell signaling through adhesion receptors, regulates the concentration of soluble factors, and serves as an additional barrier for transport. However, our understanding of the composition of BM remains incomplete. Here we analyze recent proteomic and genomic data to assess the composition of BM in human and mouse brain, and in tissue-engineered BBB models. All data sets confirm that the main components of brain BM are collagen IV a1/2 and laminin, along with agrin, perlecan, and nidogen. Transcriptomic data from human BMECs suggests that the main laminin isoform is Laminin 321, while transcriptomic data from mice and proteomic data from mice and humans suggest that Laminin 521 is the predominant isoform. Transcriptomic data from iBMECs suggest that Laminin 511 is the predominant isoform. The supporting molecules agrin, perlecan, and nidogen were detected at significant levels in all studies, although only nidogen 1 was detected in the human transcriptomic data sets. No significant differences in human BM composition were observed in BMECs along the arterio-venous axis, or in comparison of healthy and AD brains.

Indexed as

Basement MembraneBlood-Brain BarrierBrainProteomeTranscriptomeAgrinAnimalsCollagen Type IVEndothelial CellsHeparan Sulfate ProteoglycansHumansLamininMembrane GlycoproteinsMiceProtein IsoformsProteomicsAgrinCollagen Type IVHeparan Sulfate ProteoglycansLamininMembrane GlycoproteinsnidogenperlecanProtein IsoformsProteomeBasement membraneBlood-brain barrierCollagen IVLaminin

Identifiers

PMID40294830
PMCPMC12180614

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.