ArticleVeterinary medicine and science2025
Improving Ram Semen Low Cryotolerance by Replacing the Seminal Plasma With That of High-Cryotolerant Rams or Extender.
Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Global Proteomic Analysis of Sperm and Seminal Plasma From Tropically-Adapted Rams With Contrasting Parameters of Frozen-Thawed Semen.Molecular reproduction and development · 2026Article
- Effects of Supplementing Semen Extenders With Spirulina platensis Extract on Post-Thaw Ram Sperm Quality.Veterinary medicine and science · 2026Article
- Towards cervical AI of sheep with stored semen: latest insights and future developments.The Journal of reproduction and development · 2026Review
- Presence and Location of CatSper 1-4, Opioid (μ, δ and κ) and CD44 Receptors in SPERMATOZOA from AOUDAD, IBERIAN IBEX and Mouflon.Veterinary medicine and science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Ram semen cryotolerance problem continues despite intensive research. This study aimed to evaluate the effect of replacing total seminal plasma (SP) with that of high-cryotolerant rams or extender on semen quality parameters in low-cryotolerant rams. Rams previously determined as low (n = 6) and high-cryotolerant (n = 5) were used in the breeding season. High-cryotolerant rams were only included with their SP in the SP+ group. Each normospermic fresh ejaculate from low-cryotolerant rams was split into three aliquots to form the groups. The control aliquot was diluted using a two-step process. Centrifugation removed SP from the second aliquot (SP- group), and the sperm pellet was rediluted with a Tris-based extender using the same amount of SP removed. The third aliquot's SP was removed as in the SP- group, but the remaining sperm pellet was rediluted with fresh SP harvested from high-cryotolerant rams using the same amounts of removed SP in the SP+ group. Semen was rediluted with a Tris-based extender in the SP- and SP+ groups using a two-step process as in the control group. Sperm quality was similar between the groups after dilution and equilibration. However, the post-thaw semen quality was higher in the SP- (one parameter) and SP+ (four parameters) groups than in the control group (p < 0.05). Additionally, these parameters were similar in the SP- and SP+ groups. This study showed that, although an adequate extender had positive effects, replacing the whole SP with that of high-cryotolerant rams may better solve the low cryotolerance problem in ram semen.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.