Evidence map›Paper›PMID 40293439›Full record

ArticleMicrobiology (Reading, England)2025

Co-purification of the GroEL chaperone during outer membrane vesicle purification: insights from

Maude F Paquet, Steve J Charette

Abstract read
In one paragraph

Article in Microbiology (Reading, England), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Maude F PaquetInstitut de biologie intégrative et des systèmes (IBIS), Université Laval, Quebec City, Quebec, Canada.
Steve J CharetteInstitut de biologie intégrative et des systèmes (IBIS), Université Laval, Quebec City, Quebec, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Outer membrane vesicles (OMVs) are naturally produced by Gram-negative bacteria and originate from their outer membrane. They can be extracted using ultracentrifugation or ultrafiltration using concentration columns, followed by purification with a density gradient. However, these methods may co-purify contaminants with similar physical properties. Several studies have identified GroEL, a chaperonin, as a major protein in OMV preparations. Using

Indexed as

Aeromonas salmonicidaBacterial Outer MembraneBacterial Outer Membrane ProteinsChaperonin 60Mass SpectrometryMicroscopy, Electron, TransmissionMolecular ChaperonesBacterial Outer Membrane ProteinsChaperonin 60Molecular ChaperonesAeromonas salmonicida subsp. salmonicidaAmicon® columnelectron microscopyGroELoptiprep gradientouter membrane vesicles (OMVs)purification

Identifiers

PMID40293439
PMCPMC12038026

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.