Evidence map›Paper›PMID 40291718›Full record

ArticlebioRxiv : the preprint server for biology2025

RAPID-DASH: Fast and Efficient Assembly of Guide RNA Arrays for Multiplexed CRISPR-Cas9 Applications.

Asfar Lathif Salaudeen, Nicholas Mateyko, Carl G de Boer

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Asfar Lathif SalaudeenGenome Science and Technology Graduate Program, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4.ORCID 0009-0004-8447-6141
Nicholas MateykoGenome Science and Technology Graduate Program, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4.ORCID 0009-0003-6601-5520
Carl G de BoerSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada, V6T 1Z3.ORCID 0000-0001-8935-5921

Funding

Proteome-wide base editor screens to assess phosphorylation site functionality in immunosenescenceR01CA279795 · NCI · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI Samuel Anthony Myers · 2024 to 2026
$1.7M
NCI NIH HHS R01 CA279795
6 · The paper itself

Abstract

Guide RNA (gRNA) arrays can enable targeting multiple genomic loci simultaneously using CRISPR-Cas9. In this study, we present a streamlined and efficient method to rapidly construct gRNA arrays with up to 10 gRNA units in a single day. We demonstrate that gRNA arrays maintain robust functional activity across all positions, and can incorporate libraries of gRNAs, combining scalability and multiplexing. Our approach will streamline combinatorial perturbation research by enabling the economical and rapid construction, testing, and iteration of gRNA arrays. To facilitate the adaptation of this approach, we have made a web tool to design oligo sequences necessary to assemble gRNA arrays.

Indexed as

CRISPR-Cas9genome engineeringGolden Gate AssemblygRNA Arraymultiplexingpolymerase cycling assembly

Identifiers

PMID40291718
PMCPMC12027327

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.