Evidence map›Paper›PMID 40291653›Full record

ArticlebioRxiv : the preprint server for biology2025

SpeedyTrack: Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.

Megan A Steves, Ke Xu

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Megan A StevesDepartment of Chemistry, University of California, Berkeley, Berkeley, California 94720, United States.
Ke XuDepartment of Chemistry, University of California, Berkeley, Berkeley, California 94720, United States.ORCID 0000-0002-2788-194X

Funding

Intracellular transport and organelle biology at the nanoscale: A multidimensional super-resolution approachR35GM149349 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Ke Xu · 2023 to 2026
$1.8M
NIGMS NIH HHS R35 GM149349
6 · The paper itself

Abstract

We introduce spatially-encoded dynamics tracking (SpeedyTrack), a strategy to enable direct microsecond wide-field single-molecule tracking/imaging on common microscopy setups. Capitalizing on the native sub-microsecond vertical charge shifting capability of popular electron-multiplying charge-coupled devices (EM-CCDs), SpeedyTrack staggers wide-field single-molecule images along the CCD chip at ~10-row spacings between consecutive timepoints, effectively projecting the time domain to the spatial domain. Wide-field tracking is thus achieved for freely diffusing molecules at down to 50 μs temporal resolutions for >30 timepoints, permitting trajectory analysis to quantify diffusion coefficients up to 1,000 μm

Identifiers

PMID40291653
PMCPMC12026894

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.