Evidence map›Paper›PMID 40291419›Full record

ArticleBio-protocol2025

A Miniaturized Percoll Gradient Method for Isolation of Quiescent Cells of Yeast.

Cenxuan Zu, Jia Dong, Yue Zhang, Yao Zhang, Zongzheng Huang, Shaolan Zou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Cenxuan ZuTianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin, China.
Jia DongTianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin, China.
Yue ZhangTianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin, China.
Yao ZhangSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
Zongzheng HuangTianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin, China.
Shaolan ZouTianjin R&D Center for Petrochemical Technology, Tianjin University, Tianjin, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quiescence, the temporary and reversible exit from proliferative growth, is a fundamental biological process. Budding yeast is a preeminent model for studying cellular quiescence owing to its rich experimental toolboxes and evolutionary conservation across eukaryotic pathways and processes that control quiescence. Yeast quiescent cells are reported to be isolated by the continuous linear Percoll gradient method and identified by combining different features such as cell cycle, heat resistance, and cell morphology (single cell). Generally, 10-25 mL of Percoll isotonic solution is first obtained by mixing Percoll with NaCl in 12.5-30 mL centrifugal tubes. Then, the gradient is prepared at high speed for 15-60 min. Finally, approximately 2 × 10

Indexed as

2 mL centrifugal tubeCell cycleCell densityDensity gradient beadDIC imagingHeat resistanceMiniature continuous linear Percoll gradientQuiescent cellUnicellular microbeYeast

Identifiers

PMID40291419
PMCPMC12021590

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.